摘要
目的探讨E26转录因子-1(E26ts-1)、基质金属蛋白酶-1(MMP-1)及其抑制剂(TIMP-1)在脉络膜黑色素瘤中的表达及其与肿瘤浸润及转移的关系。方法以免疫组织化学法检测78例葡萄膜黑色素瘤患者中的E26ts-1,MMP-1和TIMP-1的表达,并按肿瘤细胞的形态进行分型:梭型细胞型,类上皮细胞型和混合细胞型。进行临床随访,计算平均生存时间,以SPSS10.0统计软件包进行统计学处理。结果78例脉络膜黑色素瘤患者中,梭型细胞型21例,类上皮细胞型34例,混合细胞占23例。TIMP-1呈低表达,E26Ts-1和MMP-1在三种类型的脉络膜黑色素瘤中均有表达;表达强度为梭型细胞型、混合细胞型和类上皮细胞型依次递增。共回访37例患者,其中梭型细胞型18例,平均生存时间为(78.33±24.69)个月;混合细胞型10例,平均生存时间(61.44±20.46)个月;类上皮细胞型9例,平均生存时间(36.76±12.19)个月。患者生存时间与E26ts-1及MMP-1的表达强度呈负相关(P<0.01)。结论E26Ts-1和MMP-1的高表达及TIMP-1的低表达可能与脉络膜黑色素瘤的转移浸润有关。
Objective To investigate the expression of E26 transformation-specified （E26ts-1）, matrix metalloproteinase-1 （MMP-1） and tissue inhibitor of matrix metalloproteinase-1 （TIMP-1） in ehoroidal melanoma and the correlation with the tumor＇s infiltration and metastasis. Methods Immunohistochemistry was used to detect the expression of E26ts-1,MMP-land TIMP-1 in 78 cases of choroidal melanoma who were divided into shuttle-cells ,paraepithelial-cells and mixed-cells type according to the configuration of tumor cells. The patients were followed up and their average existing time was calculated. The results were statistically computed with statistic SPSS 10. 0 package. Results In the 78 cases, shuttle-cells type was found in 21, paraepithelial-cells type in 34, and mixed-cells type in 23. Expression of TIMP-lwas low in uveal melanoma,while expression of E26ts 1 and MMP-1 was obviously found in the three types of choroidal melanoma;the sequence of expression intensity was shuttle-cells, mixed-cells and paraepithelial-cells type. Among 37 cases who had been followed up,the shuttle-cells type was in 18 with the average existing time of （78. 33±24.69） months,the mixed-cells type was in 10 with the average existing time of （61.44±20. 46） months,and the paraepithelial-cells type was in 9 with the average existing time of （36.76±12.19） months. The existing time was negative correlated with the intensity of expresion of E26ts-1 and MMP 1. Conclusion The high expression of E26ts-1 and MMP- 1and low expression of TIMP-lmay relate to the choroidal melanoma＇s infiltration and metastasis.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2006年第3期174-176,共3页
Chinese Journal of Ocular Fundus Diseases
关键词
脉络膜肿瘤
黑色素瘤
转录因子
基质金属蛋白酶
蛋白酶抑制剂
Choroidal neoplasms
Melanoma
Transcription factors Matrix metalloproteinases
Protease inhivitors
