摘要
用核酸外切酶Ⅲ和s1核酸酶,逐步缺失能在原核细胞中有效表达人乳瘤病毒16型E_7开放读码框的质粒P16E_7TNP1,获得了一系列DNA逐渐缩短的质粒。它们能在大肠杆菌中表达产生一组从羧基端往氨基端逐步减少的融合蛋白,用Western blot方法检测相应融合蛋白与阳性血清的免疫反应。分析实验结果,编码免疫反应表位的核酸序列于619至644之间。
To map the immunoreactive epitope of HPV 16 ORF, the pro-caryotic expression plasmide p16E7NP1 was deleted with exonuclease Ⅲ and nuclease S1. The deleted plasmids, which contained a nested set of 3' to 5' deletion in the HPV 16 E7 ORF, expressed a set of carboxy to amino terminus truncated fusion proteins. Region containing the immunoreactive epitope was mapped by determining which of the deleted fusion proteins retained reactivity with sera in Western immunoblot assays. The coding sequence for a human antibody-reactive linear epitope mapped between HPV16 nucleotide coordinates 619 and 664.
出处
《病毒学杂志》
CSCD
1990年第3期245-251,共7页
关键词
人乳头瘤病毒
开放读码框
表位
Human Paplliomavirus Type 16 Epitope E7 Open Reading Frame
