摘要
从VHA273病毒感染中国棉铃虫的典型病虫尸抽提纯化多角体。经碱解释放的多角体蛋白,用超速离心,Sepharose 6B柱层析纯化,多种方法鉴定其纯度。SDS-PAGE测出多角体蛋白亚基分子量MW=28,000±500d。等电聚焦电泳测出7条电泳带,其主带p15.78±0.02,次带p15.3S-6.25。氨基酸组成分析表明富含酸性氨基酸。Niles兰染脂蛋白、甲苯胺兰染糖蛋白结果均呈阳性。
Polyhedra were extracted from the cadavers of Heliothis armigera infected with VHA-273 strain of Heliothis armigera nuclear polyhedrosis virus. Observation under electron microscope showed that the polyhedra consisted of mNPV (90%) and sNPV(10%). The purity of polyhedrin purified by serial procedures was confirmed by immunological and electrophoresis methods. When the isoelectric point of the polyhedrin was determined by isoelectric focusing electrophoresis, 7bands appeared, with a major band at p15.78±0.02 and minor bands at p15.35—6.25. The molecular weight of the polyhedrin subunit is 28000±500 dalton by SDS-PAGE. Amino acid analysis showed that acidic amino acids prodominate. When stained with Nile's blue and Toluidine blue, the polyhedrin showed positive reaction.
出处
《病毒学杂志》
CSCD
1990年第3期294-299,共6页
基金
国家自然科学基金
关键词
HaNPA
病毒杀虫剂
多角体蛋白
Heliothis armigera nuclear polyhedrosis virus VHA-273 Polyhedrin
