摘要
首次利用虫体克隆技术，对野生型中国棉铃虫核型多角体病毒（ＨａＳＮＰＶＷ）的不同基因型进行了分离纯化。以较低浓度的ＨａＳＮＰＶＷ感染三龄初中国棉铃虫幼虫，病毒致死幼虫单虫收集，分别提取相应的病毒核酸，进行限制性内切酶分析，得到了ＨａＳＮＰＶ的七个不同基因型（ＨａＳＮＰＶＧ１至Ｇ７），其ＥｃｏＲＩ酶切图谱均不相同。用ＢａｍＨＩ酶切分析，七个基因型的酶切图谱只在一个片段上有显著区别：Ｇ１、Ｇ２、Ｇ３、Ｇ４ＤＮＡ的ＢａｍＨＩｈ片段大小是３．３０ｋｂ，Ｇ５、Ｇ６的这一片段的大小为３．５ｋｂ，而Ｇ７的这一片段为２．７ｋｂ。结果表明，野生型ＨａＳＮＰＶ至少包含七个不同的基因型。
Seven distinct genotypes were isolated from wild type Helicoverpa armigera single nucleocapsid NPV (HaSNPV W)by low mortality dose infection of 3rd instar H.armigera and progency viral DNAs extracted from individual cadaver were analysed by endonucleases.Each of the 7 genotypes had different EcoRI profiles.The BamHI profiles of the 7 genotypes could be distinguished by a particular fragment:the sizes of BamHI H fragment for G 1,G 2,G 3,G 4DNAs were 3.3kb,for G 5 and G 6,they were 3.5kb,and for G 7,it was 2.7kb.These results showed that the wild type HaSNPV was a mixture of at least 7 genotypic variants.
出处
《中国病毒学》
CSCD
1998年第1期83-88,共6页
Virologica Sinica
关键词
中国棉铃虫
核型多角体病毒
基因型
虫体克隆
Heliocoverpa armigera,Nuclear polyhedrosis virus,Genotypes,In vivo cloning
