摘要
目的探讨青蒿琥酯对中暑内毒素血症小鼠腹腔巨噬细胞内CD14和Toll样受体4 (TLR4)表达的影响。方法昆明种小鼠随机分为常温组、高温组、生理盐水组和青蒿琥酯组(均按60 mg/kg连续5 d腹腔注射),常温组在干球温度25℃±0．5℃、相对湿度43%±5%的条件下暴露2 h,其他组在干球温度35℃±0．5℃、相对湿度65%±5%的条件下热暴露,观察不同时间点(1、2 h)小鼠腹腔巨噬细胞内CD14和TLR 4 mRNA的表达及血浆中肿瘤坏死因子α(TNF-α)的含量。结果常温组CD14和TLR 4 mRNA表达分别为0．34%±0．047%和0．31%±0．062%；高温1 h组分别为0．53%±0．085%和0．45%±0．049%,与常温组的差异均有统计学意义(P＜0．01),并在2 h时呈现继续升高的趋势；生理盐水1 h组CD14和TLR4表达升高,但在2h时有轻度下降；青蒿琥酯1 h组CD14和TLR4的表达则分别为0．26%±0．051%和0．25%±0．084%,并在2 h有轻微的下降。各组TNF-α含量的变化呈现出与CD14、TLR 4变化基本一致的趋势。结论青蒿琥酯能明显降低内毒素信号转导通路上CD14和TLR 4的表达,减少TNF-α的产生,这可能是其抗中暑内毒素血症的机制之一。
Objective To study the effects of artesunate on CD14 and toll-like receptor 4（TLR 4） expressions in peritoneal macrophages of mice with heat stroke endotoxemia.Methods Kunming mice were randomly divided into the normal temperature group, the hypertherma group, the normal saline（NS） group and the artesunate group（ both i. p. 60 mg/kg daily for consecutive five days）. The normal temperature group was exposed to the condition of dry bulb temperature（Tdb） 25 ℃ - 0.5 ℃ and relative humidity（RH） 43 %± 5 % for 2 hours, while other groups were exposed to the condition of Tdb 35 % ± 0.5 % and RH 65 % ± 5 %. The mRNA expressions of CD14 and TLR 4 in peritoneal macrophages and concentrations of tumor necrosis factor α（TNF α） in plasma were observed in different time points（ 1 hour and 2 hour）. Results The mRNA expressions of CD14 and TLR 4 in the normal temperature group were O. 34 % ± 0,047 % and 0.31% ± 0. 062 % respectively. The expressions of two receptors at 1 hour in the hyperthermia group were significantly increased to 0.53% ± 0. 085% and 0.45% ± 0,049% compared with the normal group and kept increased at 2 hour（ P 〈 0.01 ）. The mRNA expressions at 1 hour in the NS group were significantly increased but a little bit decreased at 2 hour. The mRNA expressions of CD14 and TLR 4 at 1 hour in the artesunate group were 0.26% ± 0.051% anti 0,25% ± 0.084% respectively and a little bit decreased at 2 hour. The change of TNF-α in each group was almost consistent with the changes of CD14 and TLR 4. Conclusion Artesunate can reduce significantly the expressions of CD14 and TLR 4 in LPS signal transduction pathway and the concentration of TNF-α, which perhaps is one of the most important mechanisms that artesunate fights against endotoxemia.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第4期226-228,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
广东省自然科学基金(5004774)
