摘要
目的：采用高效液相色谱法检测发酵液中的替考拉宁含薰。方法：色谱柱为Discovery R C18流动相为乙腈-0．02mol／L乙酸铵，梯度洗脱，柱温30℃，检测波长为277nm。结果：替考拉宁的5个主要成分T-A2-1，T-A2-2，T-A2-3，T—A2—4和T—A2—5在0．2-1．0mg／ml浓度范围内线性关系良好，相关系数（r）分别为0．9996、0．9992、0．9990、0．9994和0．9993。结论：该方法具有测定方便、快捷、结果准确等特点。
Object： To develop a reverse - phase, high - performance liquid chromatography （ RP - HPLC） method for the determination of teicoplanin in fermentation broth. Methods： The separation was performed on an analytical column （ Discovery R Cis, 5μm, 25 cm× 4.6 mm ID ）. The mobile phase consisted of acetonitrile - 0.02 mol/L ammonium acetate with gradient elution. The column temperature was kept at 30 ℃ and the detection wavelength was set at 277 nm. Results： There was a good linearity over the range of 0.2 - 1 mg/ml with r of 0.999. Conclusion： The method was convenient, rapid and accurate. It was suitable for online monitoring of teicoplanin during fermentation.
出处
《天津药学》
2006年第2期21-23,共3页
Tianjin Pharmacy
