摘要
目的探讨核酸诊断方法用于广州管圆线虫诊断的可能性。方法根据广州管圆线虫成虫rRNA大亚基基因部分序列设计引物,对广州管圆线虫成虫DNA进行PCR扩增,并对该方法的敏感性和特异性进行评价。结果经琼脂糖凝胶电泳和DNA序列测定证实:PCR产物的大小和序列结构与待扩增的广州管圆线虫目的基因完全一致。结论本方法有较好的敏感性和特异性,为将其用于人体广州管圆线虫病的诊断奠定了基础。
Objective This is a feasibility study to determine if PCR could be used in the diagnosis of Angiostrongylus cantonensis （A.cantonensis）. Method DNA from adult worm of A.cantonensis was amplified with the primers specific with the large subunit ribosomal RNA gene from A.cantonensis. Specificity and sensitivity of the PCR method was also evaluated. Result The size and sequence of PCR products were consistent with the target DNA sequence of A.cantonensis. Conclusion PCR is a sensitive and specific method for the detection of A.cantonensis.
出处
《热带医学杂志》
CAS
2006年第4期414-415,共2页
Journal of Tropical Medicine
基金
广州医学院博士启动基金(No.025101)。
