摘要
目的评价广州管圆线虫Mr32000抗原(AC32)的诊断价值。方法利用切胶纯化法从广州管圆线虫成虫抗原中获取AC32,用Westernblotting和ELISA方法检测61例广州管圆线虫感染大鼠血清、5例正常鼠血清、1例广州管圆线虫病人血清、50例其他寄生虫体阳性患者血清和50例献血员血清。结果AC32和成虫粗抗原包被的ELISA检测61份感染大鼠血清和1例临床诊断为广州管圆线虫病人血清均为阳性;AC32-ELISA检测的50例献血员、20例急性血吸虫病人、11例弓形虫病人和所检测的其他寄生虫抗体阳性患者血清均为阴性。结论AC32在广州管圆线虫病的血清学诊断中具有较高的敏感性和特异性。
Objective To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32). Methods The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and otherparasitosis. Results The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100% without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis. Conclusion AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第4期380-383,共4页
Journal of First Military Medical University
基金
国家科技部十五重大科技攻关项目(2003BA712A03-07)~~
关键词
广州管圆线虫
抗原/纯化
免疫诊断
Angiostrongylus cantonensis
antigen/purification
immunodiagnosis
