摘要
目的探讨胰岛素样生长因子(insulin-like growth factor,IGF)Ⅰ、Ⅱ、血小板源性生长因子(platelet-derived growth factor,PDGF)AA、BB和角化细胞生长因子(keratinocgte growth fac- tor,KGF)在大鼠肺发育过程中的作用。方法于胚胎18、20和21 d以及出生后1、4、7、10和21 d 留取SD大鼠肺组织标本,采用免疫组织化学法和Western blot对IGF-Ⅰ、IGF-Ⅱ、PDGF-AA、PDGF- BB表达进行定位、定量检测,采用RT-PCR方法对IGF-Ⅰ、IGF-Ⅱ、PDGF-A、PDGF-B和KGF mR- NA表达水平进行半定量分析。结果 IGF-Ⅰ表达高峰期为出生后4、7和10．d;IGF-Ⅱ在胎肺阶段即有丰富表达,出生后呈下降趋势,且两者在蛋白与mRNA水平的变化情况基本一致。PDGF-A、 PDGF-B mRNA在胎肺期即有明显表达,除出生后7 d时升高外(0．97±0．23,P<0．01),其余各时间点间PDGF-A mRNA表达差异无统计学意义(P>0．05);PDGF-B mRNA表达在生后4 d时有所增强(0．42±0．16,P<0．05),10 d时有所下降(0．12±0．02,P<0．05),其余各时间点间差异均无统计学意义(P>0．05)。Western blot检测结果提示PDGF-AA在胎肺组织有较强表达,出生后1、4和7 d 为其高峰期;而PDGF-BB在胎肺期表达较弱,出生后4、7和10 d时达到峰值。KGF mRNA表达以胎肺组织最强,出生后各时间点间无明显差异(P>0．05)。结论肽类生长因子表达动态改变在肺组织正常发育过程中扮演重要角色。
Objective To investigate the role of insulin-like growth factor （IGF）-Ⅰ , Ⅱ , platelet-derived growth factors （PDGF）-AA,-BB and keratinocyte growth factor （KGF） in various stages of the pulmonary development in rats. Methods All lung tissues of fetal and neonatal rats were collected at the gestational age 18, 20, 21 d, and 1, 4, 7, 10 and 21 d after birth. The expression of IGF-Ⅰ, IGF-Ⅱ , PDGF-AA and PDGF-BB was detected by immunohistochemistry and Western blot, respectively. The levels of IGF- Ⅰ , IGF-Ⅱ , PDGF-A, PDGF-B and KGF mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR）. Results The peak expression of IGF-Ⅰ was at 4, 7 and 10 d after birth. IGF-Ⅱ was detectable early in fetal lung development and decreased gradually until 21 d after birth. The changes of IGF-Ⅰ , Ⅱ mRNA were similar to IGF-Ⅰ , Ⅱ polypeptides. The PDGF-A,PDGF-B mRNA were abundant early in fetal lung development. The steady state of PDGF-A chain mRNA was significantly different only at 7 d （0.97±0. 23 ,P〈0.01）. The PDGF-B chain mRNA was increased at 4 d in some degree and decrease slightly at 10 d （0. 42±0. 16 and 0.12 ±0.02,P〈0. 05）, but no differences at other time points were found （P〉0. 05）. The PDGF-AA polypeptide was abundant early in fetal lung development, and the expression peak was found in 1, 4 and 7 d after birth. KGF mRNA was higher in the fetal samples than that of rats after birth, but no difference in postnatal rats at all time points. Conclusions Peptide growth factors may play a critical role in the development of lung in rats.
出处
《中华围产医学杂志》
CAS
2006年第2期117-121,T0003,共6页
Chinese Journal of Perinatal Medicine
基金
国家自然科学基金资助项目(30471824)
