摘要
目的采用超临界CO2流体萃取法及超声波溶剂萃取法从千年健中提取挥发油,用气相色谱-质谱联用技术对其化学成分进行分析。方法采用超临界CO2流体萃取法与超声波溶剂萃取法从千年健中提取挥发油,采用不同类型的毛细管柱进行分析,找出最佳条件,用归一化法测定其百分含量。并用气相色谱-质谱法对化学成分进行鉴定。色谱条件:DB-5毛细管柱(30 m×0.25 mm,0.25μm),柱温60℃(8 m in)4℃.m in-1→250℃(30 m in);分流进样,分流比1∶50;进样口温度280℃。结果超临界CO2流体萃取法提取的挥发油共鉴定了54种成份,占挥发油总成分的93%以上;超声波溶剂萃取法提取挥发油共鉴定了51种成份,占挥发油的89%以上。结论超临界CO2流体萃取法提取的挥发油比超声波溶剂萃取法能更真实、全面的反映药材中的化学成分。
Objective The essential oils were extracted from Homalomena occudh（ Lour. ）Schott. by supercritical fluid extraction and ultrasonic solvent extraction. The chemical components of essential oils were analyzed by GC/MS. Methods The essential oils were extracted from Homalomena occudh（ Lour. ）Sehott. by supercritical fluid extraction （SFE） and ultrasonic solvent extraction, the components were analyzed with different kinds of capillary columns, the optimum separated and analytical conditions were studied and the amount of the components were determined by normalization method. The components separated were identified by GC/MS. Chromatographic conditions were ： Capillary columns DB-5 （30 m×0.25 mm, 0. 25μm） ; Column temperature 60℃（8 min） 4℃·min^-1→250℃ （30 min） ; Split injection, split ratio 1： 50; Injector temperature 280℃. Results There were 54 components from Homalomena occudh（ Lour. ） Schott. by supercritical fluid extraction that composed of about 93% of the total components were separated and identified; There were 51 components from Homalomena occudh （Lour.）Schott. by ultrasonic solvent extraction that composed of about 89% of the total components were separated and identified. Conclusion The result got by SFE method can reflect the chemical components of the medicinal materials more accurately and more comprehensively.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第4期533-536,共4页
Lishizhen Medicine and Materia Medica Research
基金
山东省教育厅高校自然科学基金资助项目(No.200421006)
