摘要
目的研究石菖蒲、赤芍醇提取物对实验性抑郁及脑肠肽的影响。方法通过小鼠悬尾法、游泳绝望法研究受试药物对实验性抑郁行为的影响;建立未预知慢性应激所致大鼠抑郁模型,采用放射免疫方法分别检测血浆、结肠和垂体的血管活性肠肽(VIP)和P物质(SP)含量变化,研究其对中枢性与外周性脑肠肽的影响。结果10min自发活动计数表明,药物不影响动物的自主活动。石菖蒲、赤芍醇提物400mg/kg能显著缩短悬尾和游泳的不动时间;400mg/kg表现出对5-HTP毒性显著增强;200mg/kg、400mg/kg均能显著增强亚致死剂量NA的毒性,导致动物死亡率升高。320mg/kg能显著升高未预知慢性应激所致抑郁大鼠血浆、结肠、垂体VIP含量和结肠SP含量;降低血浆和垂体SP含量,无统计学意义。结论石菖蒲、赤芍醇提物对实验性抑郁具有抑制作用,可对未预知慢性应激所致脑肠肽VIP、SP含量变化产生明显影响,从而提示抗抑郁作用机制与此有关,并为该类药临床治疗抑郁症伴有胃肠道症状提供了依据。
Objective： To explore the effect of Rhizoma Acori Tatarinowii and Radiz Paeoniae Rubra Extract on experimental depression and brain - gut peptide. Methods ： Observe the effect on experimental depression by mice tail - hanging test and mice forced swirmning test; setup rats depression model hy unpredictable chronic stimulation, . Determine the content of VIP and SP in plasma, colon and pituitary by the method of radioimmunity, to re,arch the effect on central and periphery brain - gut peptide. Results： The data in 10 minutes spontaneous activity indicates that the ethanolic extract does not disturb the spontaneous activity of the animal.400mg/kg dosage of ethanolic extract can obviously shorten the immobility time in tail - hanging test and lowed swimming test; the 400mg/kg dosage show a eubanced toxicity on 5 - HTP; both dosage of 200mg/kg, 400mg/kg can obviously eubance the toxicity of sub - lethiferous dosage NA, thus induce a higher mortality. The 320mg/kg dosage can obviously increase the VIP content in rats plasma, colon and pituitary and the SP content in colon, under the unpredictable chronic stimulation; Meanwhile, the drug also decease the SP content in plasma and pituitary, which has no meaning in statistics. Conclusions： The ethanolic extract can inhibit the experimental depression and affect the unpredictable chronic stimulation induced content change of VIP and SP; which has provided more choices in clinical therapy on depression go with gastrointestinal syndrome.
出处
《华西医学》
CAS
2006年第2期321-322,共2页
West China Medical Journal
关键词
石菖蒲
赤芍
醇提物
抑郁症
血管活性肠肽
P物质
Rhizoma Acori Tatarinowii
Radiz Paeoniae Rubra
ethanolic extract
depression
vasoactive intestinal polypeptide
substance P
