摘要
目的探讨微血管密度(MVD)和血管内皮生长因子(VEGF)在膀胱癌组织中的表达及临床意义。方法应用免疫组化S-P法检测40例膀胱癌组织和10例正常膀胱组织中MVD和VEGF的表达。结果40例膀胱癌组织中MVD记数为MVD为(47.9±8.7)。其中Ⅰ级、Ⅱ级和Ⅲ级膀胱癌MVD分别为(36.2±7.5)、(44.4±9.7)和(54.9±7.0);VEGF阳性率72.5%(29/40),Ⅰ级、Ⅱ级和Ⅲ级阳性率分别为43.8%(7/16)、85.7%(12/14)和100%(10/10)。正常膀胱组织MVD(13.4±7.8),VEGF表达为0%。结论MVD值和VEGF的表达与膀胱癌的病理分级及临床分期有关,可以作为膀胱癌诊断和预后的有效指标之一。
Objective： To study the expression of vascular endothelial growth factor （VEGF） and microvessel density （MVD） in Carcinoma of urinary bladder and its clinieel significance. Materials and Methods： With mouse anti - human VEGF and CD34 monoclonal antihody, immunohistoehemistry method （S - P） was used to examine the expression of VEGF and MVD in 40 cases of transitional cell carcinoma （TCC）. All assays were conducted according to the manufactures＇ guidelines. Results： All Forty patients had histologically confirmed TCC, The VEGF was widely expressed in the cytoplasm of endothelial cells and tumor cells of hladder carcinoma. The expression rate of VEGF was 82.5%. The level of VEGF protein in endothelia and endothelial cancer cells of grade Ⅱ and Ⅲ tumor tissues was higher than that in grade Ⅰ （ P 〈 0.05）. The count of the microvascular density （MVD）. The bladder tissues from 40 eases was （47.9 ± 8.7）. The microvascular density in grade Ⅲ （54.9 ± 7,0） was higher than that in grade Ⅱ（44.4± 9.7）, and gradeⅠ （36.2 ±7.5, P 〈 0.05）. The higher the grade and stage of the tumor, the higher the VEGF and MVD. Conclusions： The VEGF expression reflected the ability of the tumor growth and invasion on the hasis of angiogenesis and proliferation, respectively. The expression of VEGF and MVD in bladder tissues were significantly related with histopathological grade, stage and prognosis of TCC.
出处
《华西医学》
CAS
2006年第2期237-238,共2页
West China Medical Journal
