摘要
目的探讨根据体重监测建立一种理想的大鼠肝硬化模型的方法。方法90只大鼠随机分成3组,1组为对照组,2组按传统的方法诱导肝硬化的模型,3组按逐步增高四氯化碳和乙醇浓度的方法诱导肝硬化的模型。观察3组大鼠的体重变化,门静脉压力的变化,死亡率和肝假小叶形成率。结果1组大鼠体重明显增加,门静脉压力无变化,无死亡,无肝硬化形成。2组大鼠体重早期明显下降,后期缓慢增加,门静脉压力明显升高,死亡8只,死亡率26.7%,假小叶形成率66.7%。3组大鼠早期体重缓慢下降,后期缓慢增加,门静脉压力明显升高,死亡3只,死亡率10%,假小叶形成率86.6%。结论逐步增加四氯化碳及乙醇的浓度,并食用白酒代替乙醇可以缩短肝硬化形成的时间,提高形成率,降低死亡率。
Objective To probe an reliable teehniciue to induce cirrhotic model in rats by monitoring weight changes. Methods 90 male SD rats were randomly divided into three groups. Group Ⅰ was control group, and were treated with no CCl4 and eatable alcohol. Group Ⅱ were treated with traditional means. Group Ⅲ were treated with CCl4 and eatable alcohol. The concentration of CCl4 and eatable alcohol were increased stage by stage in a certain range during the experiment. The changes of weight and PVP, the formation rate of liver cirrhosis false lobe and mortality in three groups were observed. Results After being indused with above means of 8 weeks, the rat mortality in group Ⅰ was 0.group Ⅱ was 26.7%, group Ⅲ was 10% The formation rate of liver cirrhosis false lobe in group I was 0,group Ⅱ was 66.7%, group Ⅲ was 86.6 %. Conclusion The cirrhotic model in rats were induced by moderately increasing the concentration of CCl4 and adopting eatable alcohol in stead of ethanol showed a low mortality and high success rate.
出处
《肝胆外科杂志》
2006年第2期136-137,共2页
Journal of Hepatobiliary Surgery
关键词
大鼠
肝硬化
动物模型
四氯化碳
Rats
Cirrhosis
Animal model
Carbontetrachloride
