摘要
在一氯乙酸-氢氧化钠缓冲溶液(pH 3.0)和乳化剂OP的存在下,以二甲酚橙-邻菲罗啉为混合显色剂,Fe3+-二甲酚橙-邻菲罗啉可在水相中形成稳定的深红色配合物,该配合物在565nm处有一最大吸收波长,摩尔吸光系数为6.6×104 L/moL.cm,Fe3+含量在0.0～8.0μg/mL范围内服从朗伯比尔定律,检出限为8.48×10-4μg/mL,加标回收率为96.0%～101%。该方法用于啤酒中微量Fe3+的测定,不需将Fe3+还原为Fe2+,不需对啤酒样品进行前处理,方法简便。
Under the conditions of the existence of monochloroacetic acid-sodium hydroxide buffer solution （pH value as 3.0） and emulsifying agent OP and with xylenol orange-phenanthroline used as mixing color-developing agent, Fe3^＋- xylenol orange-phenanthroline formed stable crimson compounding agents in water phase and such compounding agents presented the highest absorption wavelength at 565 um with molar absorptivity as 6.6 × 10^4 L/moL-cm, and Fe3＊ content within the range of 0.0-8.0μg/mL was subject to Lambertbeer law with detection limit as 8.48×10^-4μg/mL and the percent recovery as 96.0 %-101%. Such method could be used in Fe^3＋ detection in beer and was simple to operate （it was unnecessary to deoxidize Fe^3＋ into Fe^2＋ and no pretreatment of beer samples was required in the detection）.
出处
《酿酒科技》
北大核心
2006年第5期98-99,共2页
Liquor-Making Science & Technology
