摘要
The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a major role in stabilizing the complex. The effect of silicotungstic heteropolyacid on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy.
The interaction between silicotungstic heteropolyacid and bovine hemoglobin （BHb） was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant Ks and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a mation of BHb was major role in analyzed stabilizing the complex. The effect of silicotungstic heteropolyacid on the conforusing synchronous fluorescence spectroscopy.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2006年第5期895-899,共5页
Chinese Journal of Inorganic Chemistry
基金
江苏省滩涂生物资源与环境保护重点建设实验室资助项目(No.JLCBE05032)
关键词
硅钨杂多酸
牛血红蛋白
紫外光谱
荧光光谱
热力学参数
silicotungstic heteropolyacid
BHb
UV-Vis spectroscopy
fluorescence spectroscopy
thermodynamic parameters
