摘要
选取奶牛体内生产胚胎93枚(其中囊胚54枚,桑椹胚39枚),比较研究了不同冷冻方法和保护剂对其的保护效果。结果表明:乙二醇处理组对奶牛囊胚的保护效果优于One-Step-Freezing组、甘油组和玻璃化组(回收率、形体正常率和继续发育率四组分别为91.0%、82.0%、66.6%;91.0%、91.0%、88.8%;86.6%、85.0%、83.3%和80.0%、66.7%、50.0%),其中继续发育率在乙二醇组、甘油组和玻璃化组间差异显著(P<0.05);而桑椹胚的各项指标以乙二醇处理组最高,玻璃化组最低,但各组间差异不显著。结果说明乙二醇处理组对胚胎的保护效果优于其它几个处理组。
The purpose of this study was to compare the preservation effects of different freezing methods to embryos in diary cows, total of 93 embryos were used（including 54 blastulas and 39 morules ）. The result showed that among the traditional freezing procedure, the morphological normal rate of blastulas in Ethylene glycol group is the highest among three groups（91.0%, 85.0% and 82.0% respectively） as well as the development rate（91.0%,82.0% and 66.6% respectively）, compared to glycerol and one-step-freezing. Two rates in vitrification were lower than traditional freezing group（66.7% and 50 % respectively）and the difference is significant. The morphological normal rates of frozen-thawed morulaes in three groups are 88.9% ,87.5% and 77.8% respectively, and the development rate in one-step-freezing group is lowest compared with others（75.0% ,85.7% and 85.7% respectively）. In vitrification group, all rates are still lower than traditional freezing methods.
出处
《家畜生态学报》
2006年第1期29-32,共4页
Journal of Domestic Animal Ecology
基金
甘肃省农业生物技术研究及应用开发项目
编号:GNSW-2004-07
关键词
奶牛
胚胎冷冻
玻璃化
乙二醇
Dairy cow
Embryo Cryopreservation
Vitrification
Ethylene glycol
