摘要
本实验研究了GMP法(glassmicropipette)冷冻保存小鼠胚胎的主要技术环节,并与目前常用的OPS(openpulledstraws)法比较了冷冻后囊胚率.实验表明,EFS30(87.71%)保护作用比EFS40(81.67%)好,但无显著差异(P>0.05);10%EG(91.37%)预处理优于5%EG(85.93%)、20%EG(88.70%),但差异不显著(P>0.05);玻璃化时间10～30s(89.47%、86.79%)显著优于(P<0.05)60s(64.00%).GMP法与OPS解冻后囊胚率分别为94.02%和92.06%,差异不显著(P>0.05),但GMP法稍好于OPS法.同时,GMP法操作比OPS法更简便.
The purpose of this study was to investigate the use of glass micropipettes （GMP） as vessels for vitrification of mouse embryos , and to compare the ratio of the blastocyst cultured in vitro with those cooled in open pulled straws （OPS）. The vitrification tset showed that EFS30 （ 87.71% ） was better than EFS40 （ 81.67 % ）, but the difference wasn＇ t significant （ P 〉0.05）, In equilibration test, 10 % EG（91.37 % ） was better than 5 % EG （ 85.93 % ） , 20 % EG （88.70 % ）, but there were no significant difference（ P 〉 0.05）. The vitrification time 10～30 s was significant better than 60 s. Both GMP（94.02% ） and OPS（92.06% ） vitrification methods can provide high rates of embryos survival, though there were no significant difference. The GMP method is easier in practice.
