摘要
目的探讨肺癌病人胸腔积液中MAGE-3基因的表达情况,为胸腔积液良恶性的鉴别提供新的诊断线索,进一步为恶性胸腔积液的基因治疗和肿瘤免疫治疗提供研究基础。方法选取20例肺癌合并胸腔积液病人为研究对象,无菌条件下抽取胸水20ml,立即离心取沉淀物,用TRIZOL裂解液抽提RNA,通过RT-PCR方法检测MAGE-3基因的表达情况,以结核性胸水和心衰病人胸水为对照,β-actin为内参照,同步检测基因的表达情况。结果20例肺癌合并胸腔积液病人胸水中检测到MAGE-3基因表达的有14例,阳性率70%,结核性胸水和心衰病人胸水中未检测到MAGE-3基因的表达。结论肺癌病人胸水中MAGE-3基因表达阳性率高,可作为进一步鉴别胸水良恶性的检测指标,为恶性胸腔积液、肿瘤的基因和免疫治疗的研究提供新的线索。
Objective：To investigate MAGE-3 mRNA expression in pleural effusion, and then to give the new evidence of diagnosis, gene therapy and immunotherapy of the malignant pleural effusion. Methods：20 ml of pleural effusion were obtained from 20 eases of lung cancers. Total RNA was extracted by TRIZOL and eDNA was synthesized. Reverse - transcription polymerase chain reaction（ RT - PCR） was performed to detect the MAGE - 3 gene expression, using β - actin as the control of the reaction, tuberculosis（TB） pleural effusion and heart failure eases as the control. Results： Of 20 eases of pleural effusion in lung cancers 14 （70%） were MAGE - 3 - positive and MAGE - 3 - negative in TB and heart failure eases. Conclusion： IMAGE - 3 was positive in pleural effusion of lung cancer. This could be an important evidence of the diagnosis, gene therapy and immunotherapy in malignant pleural effusion.
出处
《现代肿瘤医学》
CAS
2006年第5期563-565,共3页
Journal of Modern Oncology
