摘要
提出了一种利用RNA核酸识体和光开关化合物[Ru(phen)2dppz]2+(RU)高灵敏检测药物分子的荧光分析新方法.以托普霉素(TOB)为靶分子,检测限可达到10 nmol/L,同时该方法有较好的选择性,能在溶液中实现无需分离的实时检测.这种无标记检测方法有利于不稳定RNA核酸识体在分析检测中的应用,简化实验操作,为其它药物分子的检测提供了新思路.
Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity. In this paper, a novel luminescence detection method was developed by using RNA aptamers and a DNA light switching complex, [ Ru （phen）2 （dppz） ] ^2＋, as a signaling aptamer for the detection of drug tobramycin. The results showed that the method was highly sensitive and selective with the detection limit down to 10 nmol/L. The method was also very simple and could realize direct detection without separation in solution, and offers a new approach for the exploitation and application of aptamers in drug analysis.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第5期826-829,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20225516
90406006)资助
