摘要
试验了多种酸性染料与烟碱的反应，发现在ｐＨ＝４．５的醋酸—醋酸钠缓冲溶液中，酸性染料溴甲酚绿与烟碱能形成离子缔合物，并能被氯仿所苹取，其最大吸收在４２Ｏｎｍ处，烟碱含量在０～１２μｇ／ｍｌ范围内符合比耳定律，应用此法测定实际样品，灵敏度高、准确度好、操作简便．
A method for the determination of nicotine in tobacco was developed based on the sensitive colour reaction of bromocresol green (BCG) - nicotine (NT)system. In HAc - NaAc (pH =4.5)solution, an ionic associate of BCG and NT could be extracted into chloroform,and measured at 420urn. Beer's law was obeyed in the range of 0 to 12μg/ml for nicotine. The proposed method has been used to determine nicotine in tobacco with satifactory results.
关键词
酸性染料
烟碱
萃取
光度法
烟草
测定
bromocresol green
nicotine
extraction
spectropho-tometry
