摘要
目的:观察大脑中动脉梗塞大鼠海马胆碱乙酰转移酶和突触素蛋白表达变化,为探讨其学习记忆能力提供理论依据。方法:实验于2005-03/05在北京中医药大学老年医学研究所完成。选用健康SD雄性大鼠18只,随机分为3组,正常组、假手术组与模型组,每组6只。制备大脑中动脉梗塞模型,并于模型制备1个月后取各组大鼠脑组织,采用免疫组化技术检测胆碱乙酰转移酶和突触素表达量。结果:参加实验大鼠18只,每组6只,在模型制备过程中有6只死亡,进入结果分析数量12只,每组4只。①海马胆碱乙酰转移酶、突触泡膜素蛋白阳性表达面积:模型组眼(1004±464),(1704±672)μm2演较正常组眼(3994±939),(3619±993)μm2演和假手术组眼(3754±922),(3579±877)μm2演显著性减少(P<0.01)。②免疫组化染色可见模型大鼠海马胆碱乙酰转移酶阳性神经细胞数量明显减少,免疫染色浅,未见神经纤维染色;突触泡膜素阳性颗粒明显减少、稀疏。结论:大脑中动脉梗塞模型大鼠海马胆碱乙酰转移酶和突触泡膜素蛋白表达下降可能是其学习记忆障碍的神经生物学基础。
AIM： To observe the expression change of choline aeetyltransferase （CHAT） and synaptophysin （Syn） protein in the hippocampus of rats with middle cerebral artery occlusion （MCAO）, and provide the theoretical evidence for the discussion of the learning and memory abilities in rats.
METHODS： The experiment was done in the Institutes of Geriatrics, Beijing University of Chinese Medicine from March to May 2005. Totally 18 health male SD rats were randomly divided into three groups： normal group, sham group and model group, with 6 rats in each group. The rats in the model group were used to establish the MCAO models, and the brain tissues of rats in three groups were taken out one month later. The expression quantities of ChAT and Syn protein were detected by the immunohistochemieal method. RESULTS： There were 18 rats participated in the experiment, with 6 in each group. Six rats died in the model preparations were excluded, and totally 12 rats were involved in the result analysis, with 4 in each group.① Comparison of the positive expression areas of ChAT and Syn protein in hippocampus： the area of model group [（1 004±464）, （1 704±672） μm^2] was significantly smaller than those of normal group [（3 994±939）, （3 619±993） μm^2] and sham group [（3 754±922）, （3 579±877） μm^2] （P〈 0.01）. ② The results of immunohistochemical staining showed that the number of ChAT positive neurons in the hippocampus of model rats decreased markedly, with the shallow immunohistochemical staining and absent nerve fiber staining; The Syn positive granules reduced obviously and present sparsely.
CONCLUSION： The decreased expression of ChAT and Syn protein in the hippocampus may be the neurebiological base of the learning and memory impairment of rats with MCAO.
出处
《中国临床康复》
CSCD
北大核心
2006年第20期54-56,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家重点基础研究发展(973)计划(2003CB517104)
教育部重大科技项目(10408)~~
