摘要
采用Ｏ型血人新鲜胰腺冰冻切片作抗原，建立了血清胰岛细胞胞浆抗体（ＩＣＡ）的间接免疫荧光测定法，对８２例ＩＤＤＭ进行了血清胰岛细胞胞浆抗体检测，同时采用单克隆抗体技术，测定了３５例ＩＤＤＭ患者外周血Ｔ细胞亚群。结果病程６个月以内的ＩＤＤＭＩＣＡ阳性率为４１．７％，病程超过６个月为２２．６％；病程６个月以内的ＩＤＤＭＣＤ３，ＣＤ８阳性细胞数明显低于对照组（Ｐ＜０．０１），ＣＤ４／ＣＤ８显著高于对照组（Ｐ＜０．０１），随着病程延长，这种变化逐渐消失。结果提示ＩＤＤＭ发病早期存在自身免疫失调，对ＩＤＤＭ免疫干预治疗应在发病早期进行。
Unfixed cryostat sections of pancreas from blood group O human were used as the test substrate,the cytopklasmic islet cell antibody(ICA) in 82 cases of patients with IDDM was measuerd by standard indirect immunofluorescence technique. At same time,the peripheral blood T lymphoglocyte subsets in 35 cases of patients with IDDM were determined by means of series monoclonal antibodies. The results showed that the newly diagnosed IDDM patients were found to have higher incidence of positive serum ICA than patients who had longer than 6 months of illness(41.7% VS22.6%,), while CD3,CD8 positives cells were significantly lower than control(P< 0.01) and CD4/CD8 ratios apparently higher than control(P<0.01) in IDDM patients who had shorter than 6 months of illness. The results suggest the onset of IDDM may relate to autoimmune,and it is important to conduct the treatment of IDDM with immunosurpress as soon as possible.
出处
《潍坊医学院学报》
1996年第1期28-30,共3页
Acta Academiae Medicinae Weifang
