摘要
用５１Ｃｒ标记技术和冷冻蚀刻电镜技术对３０例慢性再生障碍性贫血（ＣＡＡ）患者和３０例健康人的红细胞（ＲＢＣ）寿命进行了测定并对其膜蛋白形态及分布进行了观察。用冷冻蚀刻电镜技术观察了ＣＡＡ患者治疗缓解的２０例。结果２６例ＣＡＡ患者ＲＢＣ寿命缩短或明显缩短，与健康人组比较，差异有高度显著性（Ｐ＜０．０１）。ＣＡＡ患者治疗前ＲＢＣ质膜ＰＦ面和ＥＦ面均见蛋白微粒形态异常、数量减少、分布不均匀。缓解后膜蛋白微粒的好转接近健康人，证明ＲＢＣ寿命与膜蛋白的形态、数量、质量及分布有密切关系。
This paper reported that the life span, morphology and distribution of the membrane protein granules of erythrocytes of 30 normal human subjects and 30 patients with chronic aplastic anemia(CAA) were measured by the 51Cr labelled method. They were observed by freeze fracture etching and trasmission sioneletron microscope.The membrane protein granules of red blood cells in 20 of the 30 cases of CAA were observed by freeze fracture etching and transmission electron microscope after basic cure or remission. The result showed that the half life of erythrocytes(RBC T1/2) was shortend, and noticeably shortend in CAA cases.There was a highly suignificant diffrerence to healthy people(P<0.01). There were morphological abnormalities :In sufferes of CAA before treatment,the amount of protein granules was reduced and poorly distributed in the protoplasmic face(PF) and the extracellular face (EF). After remission with CAA, the membrane protein granules of red blood cells improved to become similar to those of healthy people. The result proved preliminarily that membrane protein molecular morphology was closely related to the life span of the erythrocyte, and to the morphology, amount, quality and distribution of the membrane proteins of redblood cells.
出处
《潍坊医学院学报》
1996年第1期1-4,共4页
Acta Academiae Medicinae Weifang
基金
山东省教委资助
关键词
红细胞寿命
膜蛋白
再生障碍性贫血
life-span of erythrocytes
membrane protein
anaemia,aplastic
￣(51)Cr-RBC
frecze-fracture etching electron microscope
