摘要
目的探讨吻合肝动脉在大鼠部分肝移植早期损伤中的作用。方法选取雄性SD 大鼠180只,随机分成3组:全肝移植组(A组);部分肝移植组(B组);部分肝移植肝动脉化组(C 组)。在恢复血供后30 min,2、3、6、12、24 h等6个时间点分别取材,检测:血清肝功能指标;血清肿瘤坏死因子-α(TNF-α)水平;原位末端标记法(TUNEL)检测肝组织细胞凋亡;荧光半定量逆转录- 聚合酶链反应(RT-PCR)检测TNF-α和TNF-α受体在肝组织中的表达。结果各组总胆红素 (TB)、谷丙转氨酶(ALT)和谷草转氨酶(AST)峰值均出现在术后6、12 h后逐渐下降,TB、ALT和 AST峰值依次为B组>C组>A组(P值均<0．05);移植术后各组血清TNF-α表达水平在3 h时达到峰值,随后逐渐下降,峰值B组>C组>A组,其中B组峰值显著高于A和C组,而A、C组峰值差异无统计学意义(P>0．05);B组各时间点细胞凋亡数均显著多于A组和C组;B组肝组织 TNF-α表达峰值位于术后2 h,A、C组峰值位于术后3 h,B组12 h表达显著减少,A、C组术后12 h 为阴性表达。B组TNF-α受体2基因表达水平显著高于A、C组。结论吻合肝动脉可显著减轻部分移植肝早期损伤。TNF-α及其受体的表达强度与肝脏损伤程度有密切关系,可能是参与大鼠部分肝移植早期损伤过程的重要细胞因子。
Objective The aims of this study are to explore the effects of grafts injury in early stage on Liver transplantation with arterized partial Liver transplantation model. Methods Male Sprague-Dawley rata were chosen for donors and recipients, and divided randomly into 3 groups： whole graft group （group A）, partial graft group（group B）, partial graft with arterial reconstruction （group C）, separate to check on the six times on 30 minites, 2, 3, 6, 12, 24 h after transplantation, respectively. Main check index. Liver function in serum; Expression of TNF-a in serum sample using ELISA; Cellular apoptosis in hepatic tissue using TUNEL;Expression of TNF-α and TNF-α receptor in hepatic tissue using RT-PCR. Results Liver function： The sequence of the TB, ALT, AST peak values is group B 〉 group C 〉 group A （all P 〈 0.05）. The expresses of TNF-α in serum ascend obviously post-reperfusion in group A,B and C, to the peak value at 3 h, and fall-off to follow, in 12 h to 24 h, they still to keep far higher than control group. The peak value order is group B 〉 group C 〉 group A, the peak value of group B is much higher than those of group A and C, while there isn＇t significant deviation between group A and C. The apoptosis number of group B （partial graft group） at every time marking exceeds group A （whole graft group） and group C （partial graft arteriaLized group） .The highest of group B is in the 2 h, and group A, C is in the 3 h, but has no absolute difference in the the level of mRNA; Group B goes down absolutely after 6 hours, but after 12 and 24 h, group B, C are negative in expression. The gene-product of TNF-α receptor 2 is positive in group A, B, and C, the level in group B is higher than that in group A and C. Conclusion In part of non-arterial reconstruction rat model accepted Liver transplantation, the small graft is severer in injury ischemia reperfusion than that in part of arterial reconstruction rat model. The intension of expression in TNF-α has a close relation with the severity of Liver-injury, it may be one of the main cytokine of injury in graft.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第5期543-545,i0002,共4页
Chinese Journal of Experimental Surgery
