摘要
应用聚合酶链反应(PCR)技术检测了40例动脉粥样硬化性脑梗塞(ACI)患者和42名正常人 apoB 基因 XbaI 酶切位点上限制性片段长度多态性(RFLPs)。结果 ACI 组中 X^-(无酶切位点)等位基因频率为0.90,显著高于对照组的0.77(P<0.05)。正常对照组中 LDL-Ch、apoB 水平,ACI 组中 T-Ch水平 X^-X^+基因型个体明显高于 X^X^-基因型。本研究结果表明 XbaI 酶切点的基因变异可能与 ACI 发病有一定关系,但两者有关的确切机制不清楚,不能用已知脂质、脂蛋白和载脂蛋白的改变来解释。
Xbal polymorphic site of the apolipoprotein B(apo B)gene was examined by Polymerase Chain Reaction(PCR)technique in sample of 40 cases with documented atherosclerotic cerebral infarction(ACI)and 42 healthy ageematched individuals.Allele fre- quencies were compared between cases and controls,and their impact on lipid metabolism was also studied:The frequencies of X^- allele(absence of Xbal cutting site)in ACI cases were found as 0.90,which was significantly higher than 0.77 in controls(P<0.05).In group of controls genotype of X^-X^+ was associated with higher levels of LDL-Ch and apoB, and in group of ACI cases genotype of X^-X^+ associated with higher level of TCh compared with genotype of X^-X^-.Therefore,it is suggested that genetic variation at XbaI site of apo B gene may contribute to the development of ACI in Chinese.However,the mechanism by which X^- allele is correlated with ACI remains unclear,the results does not appear to be me- diated by any known disturbunce in lipids,lipoproteins and apolipoproteins.
出处
《临床神经病学杂志》
CAS
1996年第2期70-73,共4页
Journal of Clinical Neurology
关键词
动脉继样硬化
脑梗塞
载脂蛋白B基因
Atherosclerotic cerebral infarction
Apolipoprotein B gene
