摘要
AIM： To evaluate the effects of betaine on the ethanolinduced secretion of IGF-I and IGFBP-1 using radioimrnunoassay and Western blotting, respectively, in primary cultured rat hepatocytes. METHODS： Hepatocytes isolated from male SpragueDawley rats were incubated with various concentrations of ethanol and PD98059 procedures. The hepatocytes were also treated with different doses of betaine （10^-5, 10^-4, and 10^-3 mol/L）. We measured IGF-I and IGFBP-1 using radioimmunoassay and Western blotting, respectively. RESULTS： The ethanol-induced inhibition of IGF-I secretion was attenuated by betaine in a concentration-dependent manner in primary cultured rat hepatocytes. At 10^-3 mol/L, betaine significantly increased IGF-I secretion but decreased IGFBP-1 secretion. In addition, p42/44 rnitogen-activated protein kinase （MAPK） activity was accelerated significantly from 10 min to 5 h after treatment with 10^-3 mol/L betaine. Furthermore, the changes in IGF-1 and IGFBP-1 secretion resulting from the increased betaine-induced p42/44 MAPK activity in primary cultured rat hepatocytes was blocked by treatment with the MAPK inhibitor PD98059. Betaine treatment blocked the ethanol-induced inhibition of IGF-I secretion and p42/44 MAPK activity, and the ethanol-induced increase in IGFBP-1 secretion.CONCLUSION： Betaine modulates the secretion of IGF-I and IGFBP-1 via the activation of p42144 MAPK in primary cultured rat hepatocytes. Betaine also alters the MAPK activations induced by ethanol.
瞄准: 用放射性免疫测定并且西方的弄污在 IGF-I 和 IGFBP-1 的导致乙醇的分泌物上评估甜菜硷的效果，分别地在主要有教养的老鼠 hepatocytes。方法：从男 Sprague-Dawley 老鼠孤立的 Hepatocytes 与乙醇和 PD98059 过程的各种各样的集中被孵化。hepatocytes 也与甜菜硷的不同剂量被对待(10 (-5), 10 (-4), 并且 10 (-3) mol/L ) 。我们用放射性免疫测定并且分别地的西方的弄污测量了 IGF-I 和 IGFBP-1。结果: IGF-I 分泌物的导致乙醇的抑制被甜菜硷在主要有教养的老鼠 hepatocytes 以一种集中依赖者方式稀释。在 10 点(-3) mol/L，甜菜硷显著地增加了 IGF-I 分泌物，但是减少 IGFBP-1 分泌物。另外， p42/44 激活 mitogen 的蛋白质激酶(MAPK ) 活动与 10 从 10 min 显著地被加速到 5 h 术后疗法(-3) mol/L 甜菜硷。而且，在 IGF-1 和 IGFBP-1 分泌物从增加的导致 betaine 的 p42/44 MAPK 活动导致主要有教养的老鼠 hepatocytes 的变化被处理与 MAPK 禁止者 PD98059 堵住。甜菜硷处理堵住了 IGF-I 的导致乙醇的抑制分泌物和 p42/44 MAPK 活动，和 IGFBP-1 的导致乙醇的增加分泌物。结论：甜菜硷在主要有教养的老鼠 hepatocytes 经由 p42/44 MAPK 的激活调制 IGF-I 和 IGFBP-1 的分泌物。甜菜硷也改变乙醇导致的 MAPK 激活。
基金
Supported by KOSEF, project RO-2003-99-101340-0
