摘要
对神经生长因子（Nerve Growth Factor,NGF）提取纯化、活性鉴定进行了研究。将小鼠颌下腺组织匀浆、离心,取其上清,在不同pH和离子强度,依次通过两个离子交换纤维素层析柱,制得 2.5 S NGF纯品。其分子量约43.5 kD,3种主要组分的等电点分别为8.85、9.10和9.20。用鸡胚背根培养法证明其具有刺激神经生长的作用。
The nerve growth factor (NGF) was isolated from male rat maxillary glands. After homog-enization and centrif ugation of the glands, the suqernatant was sequentially chromatogaphed at various pH, on CM cellulose columns. NGF was isolated in this fashion. The molecular weight of the purfied NGF is about 13. 5 kD. The isoelectric points of three fractions of the purified product are 8. 85, 9. 10 and 9. 20 respectively. Using chicken embryo dorsal root ganglia assay, the biological activity of NGF was determined.
出处
《中国生化药物杂志》
CAS
CSCD
1996年第3期111-113,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
神经生长因子
提取
生物活性
Nerve growth factor, Isolation, Bioassay of activity
