摘要
目的:探讨壬基酚(NP)对大鼠睾丸间质细胞分泌雄激素的影响。方法:建立体外培养大鼠睾丸间质细胞原代培养体系,并采用3β-HSD染色对间质细胞进行鉴定;以不同浓度的NP(分别为较低浓度0.005、0.015、0.025、0.05、0.1μmol/L及稍高浓度0.5、5.0、10.0、15.0、25.0μmol/L)作用于间质细胞,采用3β-HSD染色观察间质细胞形态的变化,并检测间质细胞分泌睾酮含量的变化。结果:间质细胞经NP处理后,细胞形态发生变化,细胞密度降低,NP作用浓度达到25μmol/L时,细胞发生裂解。0.005及0.015μmol/L NP处理后,间质细胞睾酮分泌量增加,与对照组比较差异有显著性(P<0.05)。NP浓度达到0.5μmol/L后,睾酮分泌量与对照组相比显著下降(P<0.05),且当NP浓度为5,10,15,25μmol/L时睾酮分泌量下降极为明显(P<0.01)。结论:低浓度NP能促进间质细胞分泌睾酮,而高浓度NP抑制睾酮分泌,且高浓度NP能诱导间质细胞坏死。
Objective： To investigate the effect of nonylphenol （NP） on testosterone secretion of Leydig cells. Methods： A primary culture system of Leydig cells was set up, followed by identification of Leydig cells with 3 β-HSD staining. After treatment with different concentrations of NP, testosterone secretion was detected and morphological examination was performed. Results： Following treatment with NP, morphologic changes of Leydig cells were detected, with decreased cell density at high doses of NP. Testosterone increased at lower concentrations of NP while decreased at high concentrations of NP. Conclusion ： Lower doses of NP can stimulate the secretion of testosterone, but increased exposure to NP will inhibit testosterone secretion of Leydig cells. Besides, high concentrations of NP can cause death of Leydig cells in vitro.
出处
《中华男科学杂志》
CAS
CSCD
2006年第4期300-302,307,共4页
National Journal of Andrology
基金
国家自然科学基金(20577019)
关键词
壬基酚
雄性生殖
间质细胞
睾酮
体外研究
大鼠
Nonylphenol
male reproduction
Leydig cells
testosterone
in vitro of experiment
rat
