摘要
目的:研究体外培养大鼠睾丸Leyd ig细胞的有效方法。方法:原代培养大鼠睾丸Leyd ig细胞,用4 U/m l人绒毛膜促性腺激素(hCG)作用细胞,对照组未用hCG,放射免疫法测定培养液中睾酮浓度,3β羟类固醇脱氢酶(3β-HSD)免疫组化染色观察睾丸Leyd ig细胞形态和生物学特性。结果:培养细胞成分均一、增殖旺盛、分化率高。接种72 h后大鼠睾丸Leyd ig细胞纯度达95%。接种后24 h内,hCG刺激组较对照组睾酮分泌量明显提高(P<0.05)。结论:体外培养的睾丸Leyd ig细胞可分泌高浓度的睾酮;睾丸Leyd ig细胞的纯化和培养方法的建立,可为中老年男性雄激素部分缺乏综合征睾酮替代治疗的基础和临床研究提供一条可行的思路。
Objective： To establish a primary culture method of rat testis Leydig cell. Methods： The primary rat Leydig cells were treated with or without 4 U/ml human chorionic gonadotropin （hCG） , and testosterone in culture medium was detected by radioimmunoassay. The morphology and biological characteristics of Leydig cell were observed. Results： The culture cells were highly homogeneous, proliferative and had a high differentiation rate. The high purified Leydig cells were verified by their dynamic morphological changes and 3β-hydroxysteroid dehydrogenase△4-△5 isomerase （3β-HSD） histochemical staining. The testosterone secretion induced by hCG significantly increased （P 〈 0.05 ） 24 hours after inoculation than that induced without hCG in the control. Conclusion： It suggests that the Leydig cell cultured in vitro may secrete high concentration of testosterone, and this study laid the basis of androgen replacement therapy for partial androgen deficiency in aging male.
出处
《中华男科学杂志》
CAS
CSCD
2006年第1期14-17,共4页
National Journal of Andrology
基金
广东省科技计划基金资助(2003C34008)
广州市科委科研基金资助(2000-J-002-02)
