摘要
目的探讨注射用蒺藜皂苷(GSTT)对大鼠实验性脑出血(intracerebral hemorrhage,ICH)的脑保护作用。方法建立血淤模型,1d后应用大脑立体定位技术,自大鼠尾动脉取不抗凝血液50μl缓慢注入尾状核;建立大鼠实验性脑出血模型,观察其脑出血后脑水肿的变化及其行为学的影响;检测大鼠不同切变率的全血黏度,检测大鼠血浆及脑组织匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及脑组织匀浆中一氧化氮(NO)的含量。结果GSTT可明显降低出血脑组织的脑水分含量,明显改善神经行为,降低全血黏度,并可升高血浆及脑组织匀浆中SOD活性,降低MDA含量,降低脑组织匀浆中NO含量,与模型组比较有显著差异(P<0.05或P<0.01)。结论GSTT可减轻脑出血后神经细胞损伤、降低脑水肿、改善神经行为,降低全血黏度、改善血液流变性,其抗损伤机制可能与抗自由基、降低NO毒性有关。
Objective To investigate the protective effects of Gross Saponins of Tribulus Terrestris on intracerebral hemorrhage in rats.Methods The rat model of blood stasis was estabhshed. After 1 day experimental intracerebral hemorrhage was induced in rats through infusion of 50μl of fresh autologous blood drawn from caudal artery into the caudate uncleus. The changes in brain edema and behavior were observed. Whole blood viscosity at different shear rate was evaluated.Plasma SOD and MDA,as well as brain tissue SOD,MDA and NO levels,were determined. Results GSTT significantly reduced brain water content, neurological deficits, and whole blood viscosity. In addition GSrlT significantly increased SOD content in plasma and brain tissue, decreased MDA content in plasma and brain tissue, as well as plasma brain tissue NO levels. Conclusion GSTT might attenuate the neuron injury induced by intracerebral hemorrhage via antioxidant property.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2006年第2期99-102,共4页
Journal of Harbin Medical University
基金
国家自然科学基金资助项目(30472020)
高校博士点基金支持(20050183029)
关键词
蒺藜皂苷
脑出血
脑水肿
全血黏度
Gross Saponins of Tribulus Terrestris
intracerebral hemorrhage
brain edema
whole blood viscosity
