摘要
目的分析泌乳素(PRL)腺瘤中雌激素受体(ER)和增殖细胞核抗原(PCNA)表达水平与肿瘤侵袭性的关系。方法对24例侵袭性PRL腺瘤患者(侵袭性组)和32例非侵袭性PRL腺瘤患者(非侵袭性组),采用免疫组化方法检测其腺瘤组织中ER蛋白和PCNA蛋白表达水平;应用逆转录聚合酶链反应(RTPCR)方法检测腺瘤组织中ERmRNA表达水平。结果ER蛋白在侵袭性组中23例阳性表达,积分光密度(IOD)为4935.12±1246.56,显著低于非侵袭性组(P<0.01);PCNA蛋白在侵袭性组中有18例阳性表达,IOD为8456.24±1534.56,与非侵袭性组(14例,4428.56±1426.25)比较差异有极显著性(P<0.01);RTPCR检测显示54例腺瘤患者有ERmRNA特异扩增条带,但侵袭性组表达水平降低(P<0.05)。结论ER表达降低及PCNA表达增高与肿瘤的侵袭性有密切关系,可以作为临床判断肿瘤侵袭性的分子标志。
Objective To investigate the relationships between the expressions of estrogen receptor （ ER ）, proliferating cell nuclear antigen （PCNA） in prolactinomas and tumor invasiveness. Methods The expressions of ER and PCNA proteins were examined by immunocytochemical method, including 24 invasive prolactinomas （ invasive group） and 32 non-invasive prolactinomas （non-invasive group）. The expression of ER-mRNA was detected by reverse transcription-polymerase chain raction （RT-PCR） technique. Results There were 23 cases of positive protein expression of ER in invasive group, the integral optical density （IOD） was 4955.12 ± 1246. 56, significantly lower than non-invasive group （P 〈0. 01 ）. 18 cases in invasive group showed positive expression of PCNA, the IOD was 8456. 24 ± 1534. 56 and was obviously different with non-invasive group （ 14 cases, 4428. 56 ± 1426. 25 ） （ P 〈 0. 01 ）. 54 cases existed idio-amplification strap of ER-mRNA by RT-PCR method, but the expression level decreased in invasive prolactinomas （P 〈 0. 05 ）. Conclusions Down expression of ER and up expression of PCNA in prolactinomas are correlated with invasiveness of prolactinomas, which may serve as the molecule marker of invasiveness in prolactinomas.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第2期102-104,共3页
Journal of Clinical Neurology
关键词
泌乳素腺瘤
雌激素受体
增殖细胞核抗原
prolactinomas
estrogen receptor
proliferating cell nuclear antigen
