摘要
目的:筛选并鉴定与人CD59分子特异结合的短肽,为设计具有拮抗CD59肿瘤逃逸活性的短肽封条奠定基础。方法:以高表达人CD59的中国仓鼠卵巢细胞(CHO)为靶细胞,采用竞争结合试验,对噬菌体随机12肽库进行5轮亲和筛选。用ELISA筛选噬菌体阳性克隆并对其进行DNA序列分析。结果:随机挑选的16个克隆中,有8个与人CD59的结合力高。测序后,得到3个高度同源的氨基酸序列。DNAstar分析显示,3个序列均与已公布的(PubMed)人CD2的氨基酸序列有一定的同源性。结论:获得的序列H×A××××××P××为设计肿瘤逃逸相关的CD59活性位点的短肽封条提供了技术基础。
AIM： To screen and identity the short-peptide which specifically bindto human CD59 so as to design short-peptide clamp with counteracting tumor escape activity. METHODS： Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for .5 rounds by competitive binding test, After five rounds of screening and competitive binding test, positive phage clones were screened by ELISA and sequenced, RESULTS： 8 out of 16 phage clones which were chosen randomly were identified to have high binding power to CD59. After sequencing, 3 high homologous amino acids were obtained. DNAstar analysis showed that 3 sequences had some homology with the sequence of human CD2 published by PubMed. CONCLUSION： The obtained sequenceH×A××××××P××is helpful for design of short-peptide clamp of active sites of CD59 related to tumor escape.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第2期164-166,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(No.3170893)
关键词
人CD59
肿瘤逃逸
噬菌体肽库
短肽封条
human CD59
tumor escape
phage displaypeptide library
short-peptide clamp
