摘要
目的制定和胃胶囊中延胡索乙素的含量测定方法，以更好的控制产品质量。方法采用HPLC进行测定，色谱柱为Hypersil BDS C18（5μm，4.6mm×150mm）；流动相为甲醇-0．1％磷酸（用三乙胺调节pH至6．0）（60：40）；检测波长为280mm。结果和胃胶囊中延胡索乙素的线性范围为0．104—0.728μg，r=0．9997，平均回收率为98．95％。结论该方法操作简单、重现性好、专属性高，可作为和胃胶囊含量测定的指标。
Objective To establish the determination method of Tetrahydropalmatine in Hewei Jiaonang. Method The HPLC method for Tetrahydropalmatine was used. Column ： Hypersil BDS-C18 （5 μm, 4.6 mm × 150 mm）, mobile phase ： methanol ：0.1% H3 PO4 （60 ：40）, It was detected at 280 nm. Result The linear range of Tetrahydropalmatine was 0.104 - 0. 728 μg,r =0. 9997, The mean recovery was 98.95%. Conclusion This method is simple and accurate with good reproducibility. The method could be applied to determine Tetrahydropalmatine in Hewei jiaonang.
出处
《现代食品与药品杂志》
2006年第2期13-15,共3页
JOurnal of Modern Food and Pharmaceuticals
