摘要
目的探讨醒脑启智胶囊对小鼠脑组织海马区乙酰胆碱酯酶的影响。方法120只昆明种小鼠随机分为假手术组、模型组、醒脑启智胶囊高剂量组、醒脑启智胶囊低剂量组、银杏叶组、尼莫地平组,每组20只,采用双侧颈总动脉结扎反复脑缺血再灌注的方法制备血管性痴呆小鼠模型。术后7d进行行为学实验和脑组织海马区的病理形态学观察,并用原子分光光度法测脑组织海马区乙酰胆碱酯酶活力。结果①行为学实验显示,模型组小鼠学习成绩与记忆成绩下降,各治疗组小鼠的学习成绩与记忆成绩均有提高(P<0·05或P<0·01)。②光镜下病理形态学显示,术后模型组小鼠脑组织海马区呈缺血性病理改变,各治疗组小鼠病变轻于模型组。③模型组小鼠脑组织乙酰胆碱酯酶明显低于假手术组(P<0·01),各用药组均高于模型组(P<0·05或P<0·01)。结论醒脑启智胶囊对血管性痴呆小鼠有明显治疗作用,其作用机理之一是调节脑组织乙酰胆碱酯酶的活性,减轻缺血再灌注损伤。
（ AChE ） investigate the influence of Xingnaoqizhi Capsules （XC） on acetylcholinesterase activity of hippocampal tissue in mice. Methods To divide randomly 120 mice into the shamoperated group, model group, XC groups in large and small dose, ginkgo group and nimodipine group with used 20 mice in each group. The bilateral corotid ligation and repeated cerebral ischemia reperfusion were to establish the mouse model of vascular dementia. After 7 days the ethological experiment and pathomorphism observation in hippoeampus were made and AchE activity of hippocampal tissue was detected learning 0. 01）. by and atomic spectrophotometry. Results ①The ethological experiment showed that the mouse memory ability decreased in model group, while increased in other groups （ P 〈 0.05 or P 〈 0.01） .②The result of pathomorphism with the light microscope showed that there were ischemic pathogenic changes in mouse hippocampal tissue in model group, while these changes were less serious in other groups. ③The AChE activity of model group was obviously lower than that of the sham-operated group （ P 〈 0.01 ）, and that of other groups were higher than model group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Xingnaoqizhi Capsules has a significant therapeutic effect on vascular dementia. One of this effective mechanisms is that XC can regulate AChE activity in brain tissue and relieve the injury induced by ischemia reperfusion.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2006年第3期177-180,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
河北省科技攻关计划项目(No.01276207D)
