摘要
目的:观察心脉通预防性给药对异丙肾上腺素诱导的大鼠急性心肌缺血损伤的保护作用。方法:实验于2005-11/12在暨南大学医学院中心实验室完成。①选用清洁级健康SD大鼠24只,雄雌不拘。大鼠被随机分为4组:对照组,模型组,心脉通高、低剂量组,每组6只。心脉通低、高剂量组:按大鼠体表面积换算量(10mL/kg)灌胃162,324mg/(kg·d)心脉通(用蒸馏水制成混悬液)药液。对照组和模型组:灌胃同容量生理盐水。均1次/d,连续7d。②于末次灌胃给药后30～60min,将除对照组外的大鼠麻醉后,皮下多点注射异丙肾上腺素4mg/(kg·d),连续2d,建立急性心肌缺血模型,以心电图J点偏移≥0.1mV为造模成功。③采用BL-420E生物机能实验系统检测注射异丙肾上腺素后30min和12h心电图ST-junction(J点)的位移。采用BECKMAN全自动生化分析仪,以酶法测定血清肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶活力;分别按照TBA显示法和黄嘌呤氧化酶法等方法测定血清丙二醛水平和超氧化物歧化酶活力。④多组均数比较用单因素方差分析和组间q检验。结果:大鼠24只均进入结果分析。①注射异丙肾上腺素后30min和12h,模型组心电图J点偏移程度明显高于对照组,心脉通高、低剂量组(P<0.05)。②模型组大鼠血清肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶活力明显高于对照组、心脉通低、高剂量组(P<0.05)。③模型组大鼠血清超氧化物歧化酶活力明显低于对照组和心脉通低、高剂量组(P<0.05),丙二醛水平明显高于对照组和心脉通低、高剂量组(P<0.05)。结论:心脉通药物可保护由异丙肾上腺素诱导的缺血心肌,该作用的发生与改善心肌缺血、减少自由基产生、提高抗过氧化酶活性有关。
AIM： To investigate the protective effects of xinmai tong oil acute myocardial ischemic injury induced by isoproteronol （IP） in rats.
METHODS： The experiment was completed in the Central Laboratory of Medical College of Jinan University from November to December 2005. ① Totally 24 healthy SD rats of clean grade and either gender were selected and divided randomly into control group, model group, high-dosage xinmai tong group and low-dosage xinmai tong group with 6 in each group, According to body surface area （10 mL/kg）, rats in low- and high-dosage groups were perfused with 162 and 324 mg/（kg＇d） xinmai tong, respectively, which was mixed with distilled water. Rats in control group and model group were perfused with the same volume of saline once a day for 7 successive days. ② 30-60 minutes after the last administration, rats except control group were anesthetized and injected With 4 mg/（kg· d） IP subcutaneously at various sites for 2 days to establish models of acute myocardial ischemia. Excursion at J site of electrocardiograph （ECG） ≥ 0.1 mV was regarded at successful modeling. ③ Excursion at ST-junction （J site） of ECG was detected 30 minutes and 12 hours after IP injection with BL-420E biological function experimental system; activities of serum creatine kinase （CK）, creatine kinase isoenzyme （CK-MM） and lactate dehydrogenase （LDH） were assayed with enzyme method; level of malondialdehyde （MDA） and activity of superoxide dismutase （SOD） were assayed with TBA method and xanthine oxidase method, respectively.④ Averages were compared with single-factor .analysis of variance and q test among groups.
RESULTS： Totally 24 rats entered the final analysis. ① Excursion degree at J site was higher in model group than that in control group, high- and low-dosage xinmai tong groups 30 minutes and 12 hours after IP injection （P 〈 0.05）. ② Activities of serum CK, CK.MM and LDH were higher in model group than those in control group, low- and high-dosage xinmai tong groups （P 〈 0.05）. ③ SOD activity in model group was lower thal/that in control group, low- and high-dosage xinmai tong groups （P 〈 0.05）, hut MDA level was higher than that in control group, low- and high-dosage xinmai tong groups （P 〈 0.05）.
CONCLUSION： Xinmai tong can protect myocardial ischemic injury induced by IP, which is probably related with improving myocardial ischemia,reducing production of free radical, and increasing activity of anti-oxidase.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第15期91-93,共3页
Chinese Journal of Clinical Rehabilitation
基金
教育部留学回国人员基金资助项目(2003-406)~~
