摘要
目的:观察大豆异黄酮(SIF)对高脂膳食诱导的胰岛素抵抗(IR)大鼠血清瘦素水平及白色脂肪组织瘦素mRNA表达的影响,并探讨SIF提高胰岛素敏感性的可能机制。方法:选用高脂饲料诱导的IR雄性SD大鼠,根据其胰岛素抵抗指数(IRI)随机分为模型对照组(水)和3个SIF组(50 mg／kg,150 mg/kg及450 mg／kg)。各组经口灌胃给予相应受试物1个月,空腹过夜后取材,用酶法检测各组动物空腹血糖、用放射免疫法检测空腹血胰岛素、用酶联免疫法检测血清瘦素含量,以实时定量RT-PCR法检测肾周脂肪组织瘦素基因的mRNA水平。结果:与模型对照组比较,SIF 150 mg／kg和450 mg／kg组能显著降低空腹血胰岛素水平及IRI,SIF450 mg／kg组能明显降低大鼠体重及肾周脂肪组织瘦素基因mRNA的表达、提高血清瘦素的含量。结论:SIF可能具有改善瘦素抵抗的作用,并进而提高胰岛素的敏感性。
Objective：To explore the effects of soy isoflavone （SIF） on gene expression of leptin and insulin sensibitity in insulin-resistant （IR） rats induced by high-fat, and to reveal the mechanisms of SIF in ameliorating insulin sensibitity. Methods：IR rats were randomly divided into four groups based on their insulin-resistant indexes（ IRI）： one model control group and three SIF groups that were gavaged with water solutions with SIF at doses of 0 mg/kg,50 mg/kg, 150 mg/kg,and 450 mg/kg, respectively. After one month ,fasting glucose, fasting insulin, leptin in serum, and leptin mRNA in the perirenal adipocyte were detected by enzymic method, radioimmunoassay, enzyme linked immunosorbent assay, and real time quantitative RT-PCR, respectively. Results：The model control group was used to compare against the other groups： （1）Insulin and IRI were lower in the 150 mg/kg and 450 mg/kg groups; （2） In the 450 mg/kg group ,body weight and leptin mRNA expression were lower,serum leptin content was higher. Conclusion ：These results indicate that soy isoflavone might decrease body weight of rats and leptin mRNA, increase serum leptin level, and ameliorate leptin and insulin sensitivities.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期197-200,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30030120)资助~~
关键词
异黄酮类
胰岛素抗药性
瘦素
逆转录聚合酶链反应
Isoflavones
Insulin resistance
Leptin
Reverse transcriptase polymerase chain reaction
