摘要
探讨了１５和３０天尾吊法模拟失重对大鼠肿瘤坏死因子－α（ＴＮＦ－α）的含量及骨髓细胞对ＧＭ－ＣＳＦ反应性的影响。采用放射免疫分析方法检测腹腔巨噬细胞在ＬＰＳ刺激下产生ＴＮＦ－α的含量，３Ｈ－ＴｄＲ掺入法检测骨髓细胞对ＧＭ－ＣＳＦ的反应性。结果表明，模拟失重１５天后，ＴＮＦ－α含量有下降的趋势，３０天后ＴＮＦ－α含量显著减少（Ｐ＜０．０５）。１５天后，骨髓细胞对ＧＭ－ＣＳＦ的反应性显著下降（Ｐ＜０．０１），３０天后，两组间无明显改变。结果提示，１５及３０天模拟失重降低免疫功能，ＴＮＦ－α含量的减少可能与骨髓细胞的增殖功能有关。
The purpose of this experiment was to investigate the effect of 15 and 30 d simulated weightlessness(SWL) on tumor necrosis factor-α(TNF α) production secreted by macrophage stimulated with lipopolysaccharide(LPS) and the ability of bone marrow cells to respond to granulocyte/monocyte colony stimulating factor(GM-CSF) in tail-suspension rats.The TNF α production was assessed by radioimmunoassay.The ability of bone marrow cells to respond to GM-CSF was measured by 3H-TdR incorporation method.The results showed that after 15d SWL, TNF-α production showed a tendency to decrease;and that after 30d SWL,the decrease became significant(P<0.05).The results also demonstrated that the ability of bone marrow cells to respond to GM CSF decreased significantly after 15d SWL(P<0.01);and there was no significant change after 30d SWL.It suggested that SWL could depress immune function,and that reduction of TNF α production might be rela ted to bone marrow cell proliferation function.
出处
《航天医学与医学工程》
CAS
CSCD
1997年第3期168-171,共4页
Space Medicine & Medical Engineering
关键词
模拟失重
肿瘤坏死因子
骨髓
集落刺激因子
simulated weightlessness(SWL) tumor necrosis factor-α (TNF-α) bone marrow cells granulocyte/monocyte colony stimulating factor(GM-CSF)
