摘要
目的探索鼻腔NK/T细胞淋巴瘤(N-NK/T-L)特异基因改变及其表达变化。方法对同一病例的N-NK/T-L肿瘤细胞和外周血白细胞进行限制性酶切路标基因组扫描(RLGS),并结合相应虚拟基因组扫描(VGS)生物信息分析,及基因库检索确定异常基因信号;通过对上述结果发现的异常基因进行克隆和探针制备,对3例新鲜N-NK/T-L肿瘤组织和1例外周血标本进行了Southern和斑点印迹杂交分析,并对20例石蜡组织N-NK/T-L以及对照组包括17例同部位B细胞淋巴瘤、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。结果RLGS和VGS法显示N-NK/T-L肿瘤细胞中存在T-bet基因异常;经Southern和斑点印迹杂交检测证明在3例新鲜N-NK/T-L肿瘤细胞中有T-bet基因扩增;原位杂交显示T-bet基因在20例N-NK/T-L中阳性表达者为18例(90.0%),而B细胞淋巴瘤中仅为2例(11.8%),两者比较差异有统计学意义(P<0.01)。在3例脾组织和5例慢性炎性鼻黏膜组织中均未有T-bet表达。结论T-bet基因在N-NK/T-L肿瘤细胞中存在基因扩增和过表达,T-bet基因的异常表达可能在N-NK/T-L的发生中起一定作用,并有必要对其是否可作为分类依据和辅助诊断的指标做进一步研究。
Objective To investigate the specific genetic alterations in nasal NK/T cell lymphoma （N-NK/T-L） and the significance thereof. Methods Restriction landmark genomic scanning （RLGS） was performed on the specimen of a case of N-NK/T-L and the peripheral blood leukocytes of the same case. The RLGS image was analyzed with the Virtual genome scan （VGS） software so as to discover abnormalit,） of T- bet gene. The discovered abnormal gene underwent cloning and preparation of probes. Southern blotting and dot blotting were performed on 3 cases of fresh N-NK/T-L tumor tissues and the genome of the case of peripheral blood lymphocytes. The expression of the T-bet （T-box expressed in T cell） gene in the tissue sections of 20 cases of N-NK/T-L, 17 cases of B cell lymphoma （ BCL）, 3 cases of normal spleen, and 5 cases of chronic nasopharyngitis was detected by in situ hybridization. Results RLGS combined with VGS revealed a genomic alteration in the T-bet gene and consequent Southern and dot blotting confirmed this genomie alteration in the N-NK/T-L cells. In situ hybridization showed that the T-bet mRNA expression rate of the N-NK/T-L cells was 90.0% （18/20）, significantly higher than that of the BCL （11.8% , 2/17, P 〈0. 01 ）. T-bet mRNA expression was not detected in the normal spleen and chronic nasopharyngitis tissues. Conclusion The amplification and over-expression of the T-bet gene may play a role in the development of N-NK/T-L. The potential value of over-expression of T-bet gene in diagnosis of N-NK/T-L is worth further investigating.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第13期881-885,共5页
National Medical Journal of China
基金
国家自然科学基金(30270517)
教育部教育振兴行动计划专项基金(985工程)
关键词
淋巴瘤
基因扩增
基因表达
Lymphoma
C, ene amplification
C, ene expression
