摘要
目的研究蟾蜍灵与全反式维甲酸(ATRA)联合对急性早幼粒细胞白血病(APL)原代培养细胞的诱导分化作用。方法12例初诊的APL患者的骨髓经单个核细胞分离后进行原代培养。细胞存活率测定采用锥虫蓝拒染法,细胞凋亡及诱导分化采用形态学分析、四唑氮蓝(NBT)还原试验及细胞分化抗原CD11b的流式细胞仪分解。结果蟾蜍灵5nmol/L与ATRA30nmol/L联合应用明显诱导APL原代培养细胞向成熟粒细胞分化、联合应用较单独应用ATRA时NBT还原率增加了15%～52%及CD11b表达增加了16%～69%,同时分化时间从7d缩短至4d,而且ATRA用量可减少至单用时的30%。结论蟾蜍灵与ATRA联合应用可显著增强ATRA对APL原代培养细胞的诱导分化作用。
Objective To investigate the effect of bufalin combined with all-trans retinoic acidinduced （ATRA） differentiation of acute promyelocytic leukemia （APL） cells in primary culture. Methods Fresh leukemia cells were obtained from heparinized bone marrow aspirations of 12 newly diagnosed APL patients. Cell viability was determined by trypan blue dye exclusion. Apoptosis of APL cell was assessed by morphological analysis. Differentiation of APL cell was also assessed by morphological analysis. Nitro blue tetrazolium（NBT） reduction test and expression of the granulocyte/macrophage-specific antigen CD11b was carried out with flow cytometric assay. Results Bufalin combined with ATRA can induce differentiation of APL cells towards mature stages, NBT reduction was increased 15%-52% and CD11b expression was also increased 16%-69% in combination of bufalin and ATRA as compared with that of ATRA alone , while the concentration of ATRA needed in the combination group was reduced to 30% and the time of differentiation was reduced from 7 days to 4 days. Conclusion The combination of ATRA with bufalin can siginificanfly enhance the differentiation of acute premyelocytic leukemia cells in primary culture by ATRA.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2006年第4期314-317,共4页
Chinese Journal of Internal Medicine
基金
辽宁省自然科学基金资助项目(20022077)
