摘要
目的探讨缺氧诱导因子1α(H IF-1α)的表达与血管内皮生长因子(VEGF)及微血管密度(MVD)的相关性及三者在胃癌组织中的表达与胃癌侵袭转移间的相互关系。方法对48例手术切除后的胃癌标本进行连续切片和H IF-1α、VEGF蛋白的免疫组织化学检测,用CD34单克隆抗体标记血管内皮细胞并计数MVD。结果在48例胃癌组织中,H IF-1α阳性表达32例(66.7%),VEGF阳性表达29例(60.4%),胃癌组织的MVD值为(42.5±14.7),均与正常胃黏膜组差异有统计学意义。H IF-1α的表达与VEGF的表达、MVD值呈正相关。H IF-1α、VEGF表达及MVD均值与肿瘤浸润深度、淋巴结转移及TNM分期有显著相关性。结论H IF-1α、VEGF在胃癌组织中高表达,MVD值高于正常胃黏膜。H IF-1α的表达与VEGF的表达、MVD值呈正相关。且均与与胃癌的侵袭转移密切相关。
Objective To investigate the expression of hypoxia inducible factor let （HIF-1α）, vascular endothelial growth factor （VEGF） and micro vessel density （MVD） in gastric carcinoma and to explore their correlation with clinical pathological features such as cancer invasion and metastasis. Methods Forty-eight samples of gastric carcinoma tissues were examined for the expression of HIF-1α, VEGF and CD34 by immunohistochemical method. Results The positive expression rates of HIF-1α and VEGF were 66.7% and 60.4% in gastric carcinoma respectively. The mean value of MVD was 42.5±14.7 in gastric carcinoma. The expressions of HIF-1α, VEGF and the value of MVD were significantly correlated with the depth of invasion, lymph node metastasis and TNM stage. The HIF-1α expression was positively correlated with VEGF expression and MVD value. Conclusion The overexpression of HIF-1α, VEGF and MVD consist in gastric carcinoma tissue. The HIF-1α expression is positively correlated with VEGF expression and MVD value. The overexpression of HIF-1α, VEGF and MVD value are closely related with invasion, metastasis and poor biological behavior of gastric carcinoma.
出处
《中国医师杂志》
CAS
2006年第3期309-311,共3页
Journal of Chinese Physician
关键词
血管内皮生长因子类
胃肿瘤
缺氧
毛细血管
Vascular endothelial growth factors
Stomach neoplasms
Anoxia
Capillaries
