摘要
目的:观察低氧诱导因子1α(H IF-1α)及血管内皮生长因子(VEGF)在前列腺癌(PCa)中的表达及意义。方法:32例PCa患者,根据G leason评分,将≥7分者设为高G leason评分组(n=12),<7分者为低G leason评分组(n=20)。良性前列腺增生(BPH)16例,BPH伴高级别前列腺上皮内瘤(PIN)15例,正常前列腺组织(NP)12例。采用免疫组化染色CD34观察各组组织中微血管密度(MVD)及H IF-1α、VEGF的表达情况。结果:PCa、PIN中H IF-1α阳性表达率分别为62.5%、60.0%,较BPH(6.3%)及NP(0)高,差异有统计学意义(P<0.05)。PCa、PIN中VEGF阳性表达率分别为78.1%、73.3%,较BPH(18.7%)及NP(8.3%)高,差异亦有统计学意义(P<0.05)。PCa的MVD为66.9±18.0,明显高于BPH(28.3±6.9)及NP(15.3±2.9)(P<0.05)。高G leason评分组H IF-1α、VEGF阳性率及MVD值均高于低G leason评分组,差异均有显著性(P<0.05)。结论:H IF-1α及VEGF过表达是PCa形成的早期事件,与PCa密切相关。
Objective： To study the expression of hypoxia-inducible factor 1 α （HIF-1α） and vascular endothelial growth factor （VEGF） in prostate cancer（PCa） and its biological significance. Methods： Specimens from 75 patients with PCa（32 cases）, benign prostatic hyperplasia （BPH, 16 cases）, high-grade prostate intraepithelial neoplasia （PIN, 15 cases） and normal prostate（ NP, 12 cases） were comprised in the study. The PCa was divided into high Gleason score group（ Gleason score≥7） and low Gleason score group（ Gleason score 〈 7）. Immunohistochemistry was performed with antibodies against HIF-1 α, VEGF and CD34. Microvessel density （MVD） was analyzed by staining with antibodies to CD34. Results ： The positive rates of HIF-1 α were significantly higher in PCa （62.5%） and PIN（60.0% ） than those in BPH（6.3% ）and NP（O） （ P 〈0.05）, respectively. The positive rates of VEGF were significantly higher in PCa （78.1% ） and PIN （ 73.3 % ） than those in BPH （ 18.7% ） and NP（ 8.3 % ） （ P 〈 0. 05 ）, respectively. A higher concentration of MVD was observed in PCa （ 66.9 ± 18.0 compared with those in BPH （ 28.3 ± 6.9） and NP（ 15.3 ± .9 ） （ P 〈 0 05 ）. MVD and the positive rates of HIF-1 α and VEGF were significantly higher in high Gleason score group than those in low Gleason score group.Conclusion：The results suggest tha the overexpression of HIF-1α and VEGF are closcly related to PCa.and the up-regufation of HIF-1α and VEGF are early events in pCa.
出处
《中华男科学杂志》
CAS
CSCD
2006年第1期57-59,共3页
National Journal of Andrology
关键词
前列腺肿瘤
低氧诱导因子1Α
血管内皮生长因子
微血管密度
prustatr neaplasm hypoxia-inducible factor 1α
vascular endothelial growth factor
mjcrovessel density
