摘要
目的探讨PTTG和VEGF蛋白在肝外胆管癌中的表达相关性及其与胆管癌发展的关系。方法应用免疫组化技术SABC法检测PTTG和VEGF蛋白在3 6例胆管癌标本、3 0例胆管癌旁组织和1 2例胆管良性病变中的表达。结果胆管癌组织和癌旁组织中PTTG的阳性表达率分别为7 2.2%(2 6/3 6)和6 3.3%(1 9/3 0),VEGF的阳性表达率分别为8 3.3%(3 0/3 6)和7 6.7%(2 3/3 0);在胆管良性病变组织中均未检测到PTTG和VEGF的表达。上述3种组织之间的PTTG和VEGF蛋白表达差异均有显著性(P<0.0 5)。癌组织中PTTG和VEGF蛋白的表达呈正相关(r=0.7 0 3,P<0.001)。PTTG和VEGF蛋白表达与肿瘤转移与否关系密切,但与患者的性别、年龄、肿瘤大小及分化无关。结论PTTG和VEGF在胆管癌中高表达,两者表达呈正相关;与胆管癌的转移与否关系密切。
Objective To investigate the correlation of the expressions of PTTG and VEGF proteins in extrahepatic cholangiocareinoma, and study its role in the development of extrahepatic cholangiocareinoma. Methods Expression of PTTG and VEGF proteins was detected by SABC immunohistochemical technique in 36 cases of extrabepatic cholangiocarcinoma, 30 cases of adjacent histologically noncancerous bile duct tissues and 12 cases of benign bile duct lesions. Results The positive rates of PTTG and VEGF proteins were 72.2% （ 26/36 ） and 83. 3% （ 30/36 ） respectively, in extrahepatic cholangiocareinoma ; and 63. 3% （ 19/30 ） and 76.7 % （23/30 ） in adjacent histologically noncancerous bile duct tissues. The expression of PTTG protein was significantly positively correlated with that of VEGF protein （P 〈 0. 05 ） in cholangiocareinoma. In contrast, no expression of PTTG and VEGF proteins was detectable in 12 cases of benign bile duct lesions （ P 〈 0.01 ） . The expression of PTTG and VEGF was closely related to metastasis of extrabepatic cholangiocarcionoma, but was not related to sex or age of the patients, tumor size or tumor differentiation. Conclusions There is a significant positive correlation between high expressions of PTTG and VEGF proteins in cholangiocarcinoma. The high expressions of PTTG and VEGF proteins may be related to the metastasis of extrabepatic cholangiocarcinoma.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第3期185-189,共5页
China Journal of General Surgery
基金
湖南省卫生厅科研基金资助项目(B2005011)
