摘要
构建了β-葡聚糖酶表达载体 P^(SGI)。转化枯草杆菌 SO113后,可高效表达β-葡聚糖酶,并分泌到培养基中。其酶活性约为无表达质粒的枯草杆菌的十倍。
An expression vector of Beta-glucanase gene p^(Sa1) was construc- ted.High expression level of Beta-glucanase was obtained after SO113,a B. subtilis strain,was transformed with the vector and the enzyme was secreted into the medium.The enzymetic activity was found to be tenfold as the control- led bacteria without the vector.Plasmid P^(SC1) may be used as a general expres- sion vector for the expression of other genes in B.subtilis.
关键词
Β-葡聚糖酶
枯草杆菌
表达量
载体
β-glucanase gene
construction of expression vectors
transformation of B.subtilis SO113
high expression
