摘要
以Bacillus subtilis为供体,pBR325载体,采用鸟枪法克隆了β-1,3-1,4葡聚糖酶基因bgl,并转化大肠杆菌后得到表达。结果表明,克隆子中插入的EcoRⅠ酶切片段长度为7.1kb,含有bgl基因。克隆子能合成专一地分解大麦β-葡聚糖的酶。宿主中的pBR325-bgl杂种质粒pFGl在无任何选择压力下较稳定。
Summary, A Bacillus subtilis gene (bgl) coding for a β-1, 3-1, 4-glucanasehas been transferred to Escherichia coli by the shot gun method using the plasmidvector pBR325. The gene is contained within a 7.1 kb EcoR I fragment anddirects the synthesis in E. coil of a β-1, 3-1, 4-glucanase which specificallydegrades barley glucan. The recombinant strain of Escherichia coli carrying apBR325 bgl hybrid plasmid (pFG1) is more stable without any selective pressure.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1989年第4期386-392,共7页
Journal of Fudan University：Natural Science
关键词
葡聚糖酶
大肠杆菌
基因
克隆
表达
Bacillus subtilis, Escherichia coli, plasmid,cloning
pBR325, barley glucan, β-1,3-1,4-glucanase, expression.
