摘要
以番茄、苋莱为材料进行自体嫁接和异体嫁接。观察嫁接隔离层及其两侧细胞的组织化学变化,用细胞化学定位方法比较不同嫁接组合隔离层两侧细胞中细胞色素氧化酶和多酚氧化酶活性,随嫁接组合形成,隔离层是动态变化的;两种酶活性逐渐增加到一高峰级又逐渐降低,不亲和性接嫁组合细胞中酶活性明显高于亲和性嫁接组合,且酶活性高峰出现晚2～3d。对隔离层组织化学变化和两种酶活性的动态变化与嫁接亲和性的关系作了初步讨论。
Autograft(Lycopersicum esculentum/Lycopersicum esculentum)and heterograft(Amaranthus tricolor/Lycopersicum esculentum)have been studied.The cytochemical changes,in particular about the cytochrome oxidase and polyphenol oxidase activities,of the isolation layer and in cells beside the isolation layer were observed with cytochemical localization methods.Both enzymes activities gradually rose and came down after a peak during the developmental processes of the graft unions.The enzyme activity in the incompatible heterograft union was higher than in the compatible autograft union.The peak of the enzymes activities in the incompatibje heterograft union emerged later than those in the compatible autograft union.The relationship between these changes and graft compatibility is discussed.
基金
国家自然科学资金资助课题
关键词
嫁接亲和性
酶活性
graft
compatibility
cytochrome oxidase
polyphenol oxidase
