摘要
用由毕赤酵母表达的皮蝇素A(Hypderm in A,HA)蛋白分别免疫新西兰大白兔和牦牛,了解该蛋白免疫效果。用两种不同分子量的重组HA蛋白分别免疫新西兰大白兔1次和2次,ELISA测其抗体消长规律。结果表明,3周后抗体水平达到峰值,后缓慢下降。50KD蛋白免疫效果较30KD蛋白好,差异非常显著(P<0.01)。用其中50KD的重组HA蛋白分别在7月和9月免疫自然感染牛皮蝇蛆病牦牛,观察感染率和感染强度,ELISA测其抗体消长规律。结果表明,7月免疫组与9月免疫组感染率分别为83.3%和36.3%(对照组为55.5%);平均感染强度分别为6.5和4.2(对照组为13.0),差异不显著(P>0.05)。免疫后,抗体水平上升,3个月后缓慢下降。9月免疫组免疫效果较7月免疫组好,三组抗体水平差异非常显著(P<0.01)。上述研究结果为防治牛皮蝇蛆病疫苗的深入研究和实践运用提供了参考依据。
Hypodermin A （HA） expressed by Pichia Pastoris was used to accomplish the immunoassay on rabbits and yaks to find out the immunization effect of the protein. The rabbits were vaccined with the 30 KD recombinant HA and the 50 KD protein appeared 3 days after inducement. The level of antibody reached the highest titer on the 3rd week, and came down slowly afterwards. The immunization effect of the 50 KD protein is better than that of the 30 KD one, and there is significant difference（P 〈0.01 ）. The protective effect of the 50 KD protein as a vaccine on naive yaks is quiet good. The infection rate of groups vaccined separately on July and September is 83.3% and 36.3% （55.5% as control） ; and the average infection intensity of these 2 groups is 6.5 and 4.2（ 13.0 as control）, the difference is not obvious（ P 〉0.05 ）. The level of antibody reached the highest titer on the 3rd month post vaccination, and decreased slowly afterwards. The immune effect of group vaccined on September is better than that of the group vaccined on July, the difference of the antibody level of 3 groups is very obvious （P 〈0.01 ）. This study facilitates further investigation on HA as a vaccine and the use of Pichia Pastoris in parasite domain.
出处
《中国兽医寄生虫病》
2006年第2期1-5,共5页
Chinese Journal of Veterinary Parasitology
基金
欧盟项目"HypodermosisControl"(ICA42000-30036)
