摘要
目的:观察小分子化合物丙戊酸对周围神经再生的作用。方法:实验于2005-07/10在吉林大学中日联谊医院动物实验室完成。选用成年雄性Wistar大鼠15只,随机分为假手术组、模型组和丙戊酸组3组(n=5)。模型组和丙戊酸组大鼠切断右侧坐骨神经制备单纯坐骨神经轴突切断模型,然后即刻行神经吻合术,假手术组不切断坐骨神经。丙戊酸组大鼠在神经修复术后喂食含丙戊酸水(300mg/(kg·d))16周,使血浆浓度达到50mg/L。16周后,各组大鼠取坐骨神经及双侧胫骨前肌进行组织形态学检查,观察再生的有髓神经纤维和神经再支配的肌纤维数量及大小。结果:15只大鼠全部进入结果分析。①模型组和丙戊酸组再生的单个有髓神经纤维的大小明显小于假手术组(P<0.001);模型组和丙戊酸组中再生神经有髓纤维数量是假手术组的3倍(P<0.001);丙戊酸组大鼠中有髓神经纤维数量明显高于模型组(P<0.05)。②模型组和丙戊酸组大鼠神经再支配胫骨前肌中单个肌纤维大小明显小于假手术组(P<0.05),丙戊酸组再支配胫骨前肌肌纤维数量明显高于模型组(P<0.05)。结论:丙戊酸并不能影响神经再支配肌肉组织中肌纤维的大小,但可使神经再支配肌肉中肌纤维数量显著增加,进而增强大鼠坐骨神经再生能力,提示丙戊酸对人体周围神经损伤具有潜在的临床应用价值。
AIM： To study the role of micromolecular compound valproic acid （VPA） in the regeneration of peripheral nerves.
METHODS： The experiment was conducted in the Animal Laboratory of China-Japan Union Hospital, Jilin University from July to October 2005. A total of 15 adult male Wistar rats were randomly divided into sham-operation group, model group and VPA group with 5 rats in each group. Rats in the model group and VPA group were cut off the sciatic nerve of the right side to establish simple sciatic nerve axotomy model, and then immediately conduct neuroanastomosis, while sciatic nerve of rats in the sham-operation group were not cut off. Rats in the vPA group were fed with water, in which VPA dissolved [（300 mg/（kg.d）] following neural prosthesis for 16 weeks to enable the plasma concentration reach to 50 mg/L. After 16 weeks, all rats were sacrificed for histomorphometric examination of sciatic nerves and bilateral anterior tibial muscles, and the number and size of myelinated nerve fibres and reinnervated muscle fibres were observed.
RESULTS： A total of 15 rats all entered the final analysis.（1）The size of single myelinated nerve fibre that regenerated in the model group and VPA group was remarkably smaller than that of sham-operation group （P 〈 0.001）; And the number of regenerated myelinated nerve fibres in the model group and VPA group was 3 times as that of the sham-operation group （P 〈 0.001）, and that of the VPA group was markedly higher than that of model group （P 〈 0.05）. （2）The size of single muscle fibre in reinnervated anterior tibial muscles of rats in the model group and VPA group was obviously smaller than that of the sham-operation group （P 〈 0.05）, and the number of reinnervated anterior tibial muscles was significantly higher than that of the model group （P 〈 0.05）.
CONCLUSION： VPA is unable to influence the size of muscle fibres in reinnervated muscles, whereas it can increase the number of muscle fibres, and thus reinforcing the regeneration capacity of sciatic nerve in rats, which indicates that there is a potential clinical value of VPA in applying in human peripheral nerve injury.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第14期117-119,共3页
Chinese Journal of Clinical Rehabilitation
