摘要
目的:探讨肝移植中供肝热缺血再灌注损伤发生机制。方法:建立大鼠动脉化肝移植胆道外引流模型,测定术后24h移植肝组织学、肝功能和移植肝细胞间粘附分子-1(ICAM-1)mRNA转录水平的变化。结果:随着供肝热缺血时间的延长,移植肝损伤加重,血清ALT、AST、LDH、GGT、DBIL和ICAM-1mRNA转录水平显著升高。结论:肝移植中供肝热缺血主要损伤肝细胞,血清ALT、AST、LDH是反映供肝热缺血造成肝细胞损伤的指标。ICAM-1可能是导致移植肝缺血再灌注时肝细胞损伤的主要因素。
Objective： To study the mechanism of warm ischemia reperfusion injury in graft following orthotopic liver transplantation. Methods： Orthotopic liver transplantation with hepatic artery reconstruction and biliary extra-drainage model using inbred male BN rats was established under microscopy. At postoperative hour 24, samples were collected for histopathological studies, determining the mRNA transcription levels of ICAM-1 of graft and hepatic function. Results： W.ith increasing warm ischemia of graft,hepatocytes injury aggravated and the mRNA transcription level of ICAM-1 were markedly increased. In recipient＇s serum of transplantation groups, the concentrations of AST, ALT, LDH, GGT and DBIL were significantly higher than the bile drainage group. Conclusion： Hepatocyte is the main site of warm ischemia injury associated with elevated expression of ICAM-1. It is demonstrated that serum AST, ALT, LDH could present the degree of warm ischemia injury and might be valuable markers serving as predicting index. ICAM-1 plays the key role and may be a good marker to indicate the degree of hepatocyte injure due to reperfusion injury.
出处
《天津医科大学学报》
2006年第1期8-10,共3页
Journal of Tianjin Medical University
基金
天津市自然科学基金资助(023601211)
