摘要
目的：对复方黄酮胶囊中的有效成分槲皮素进行含量测定。方法：高效液相色谱（HPLC）法，C18柱，以甲醇-0．4％磷酸（45：55）为流动相，流速为0．9mL·min^-1，检测波长为360nm。结果；槲皮素在0．3248～3．2480μg范围内与峰面积线性关系良好，r=0．9998，平均回收率为99．13％，RSD为0．78％（n=6）。结论：本方法简便，准确，重现性好。
OBJECTIVE To establish a method for determining the content of quercetin in compound flavone capsules. METHODS The suntek 18 column with methanol-0. 4% phosphorous acid （45：55） as the mobile phase was used. The flow rate was 0. 9 mL·min^-1 and the detective wavelength was 360 nm. RESULTS The linear range of calibration curve was 0. 324 8 - 3. 248 0μg,for the quercetin （r= 0. 999 8）. The average recovery was 99. 13% and the RSD was 0. 78% （n = 6）. CONCLUSION The method is simple,and accurate with a good reproducibility.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第4期386-387,共2页
Chinese Journal of Hospital Pharmacy
基金
广西壮族自治区卫生厅课题(编号:Z2003081)
