摘要
目的：建立高效液相色谱（HPLC）法检测人血浆中9-硝基喜树碱的浓度。方法：采用Kromasil C18色谱分析柱，以甲醇-磷酸盐缓冲液（48：52，pH 2．5）作为流动相，流速为1．0mL·min^-1，柱温30℃，检测波长370nm，喜树碱为内标，血浆样品用C18固相萃取小柱处理，采用加权最小二乘法计算回归方程。结果：9-硝基喜树碱的线性范围为5～100μg·L^-1，r=0．9999，最低检测限为5μg·L^-1。该方法的提取回收率为83．5％～114．4％，批间和批内RSD〈15％。结论：该方法简便、准确、重现性好且灵敏度高，适用于9-硝基喜树碱临床人体药动学研究。
OBJECTIVE To establish an HPLC method for the determination of 9-nitro-camptothecin in human plasma. METHODS A kromasil C18 column was used as analytical column. The mobile phase consisted of methanol and phosphate buffer solution（48：52,pH 2. 5）. The flow rate was 1.0 mL·min^-1 and the column temprature was at 30℃. Detection wavelength was at 3711 nm. Camptothecin was used as internal standard. Plasma samples were prepared with solid phase extraction assay. RESULTS A linear calibration curve of 9-nitro-camptothecin in plasma was obtained in the concentration range of 5 to 1000μg·L^-1（r= 0. 999 9）. The absolute recovery was between 83.5%-114. 4% and the relative recovery is between 84. 0% - 104. 9%. The intra- and inter-run precision was measured to be below 15 %. CONCLUSION The method is shown to be convenient and accurate,and suitable for clinical pharmacokinetic study of 9-nitro-camptothecin.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第4期378-380,共3页
Chinese Journal of Hospital Pharmacy
基金
国家863计划资助项目(课题编号:2004AA2Z3720)
